关键词: 甲基化硒酸, 前列腺肿瘤, 细胞周期

Abstract: To observe the effect of methylseleninic acid (MSA) on proliferation and apoptosis of the prostatic cancer cells DU145 and approach the mechanism.Methods After DU145 cells were treated with 1.25,2.50 and 5.00 μmol•L^-1MSA,the cell viability was examined by MTT test. The techniques of acridine orange staining and flow cytometry were used to analyze apoptotic cycle and calculate the apoptotic index. The expression of active caspase-3 was detected with immunocytochemistry. Results After DU145 cells were treated with 1.25,2.50 and 5.00 μmol•L^-1MSA,the growth inhibitory rates of cell line were 16.35%,38.10% and 73.54 %,respectively,the inhibitory rate increased with the increasing of MSA concentration in a dose-dependent manner. There existed obvious differences between three groups（P<0.01）.Acridine orange staining results showed that the cells were apoptotic state when treated with 1.25,2.50 and 5.00 μmol•L^-1MSA,the number of apoptotic cells increased with MSA dose increasing. Flow cytometry results showed that when the cells were treated with 1.25,2.50 and 5.00 μmol•L^-1MSA for 48 h,the apoptotic rates were 5.34%,8.71% and 13.6%, respectively; there existed obvious differences between three groups （P<0.05）.The expression of active caspase-3 was upregulated with MSA dose increasing.Conclusion MSA can induce the apoptosis of prostatic cancer cells DU145 and has significant anti-tumor effect,its mechanism may be related to activate the pathway of caspase.

Key words: methylseleninic acid, prostatic neoplasm, cell cycle