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低剂量辐射诱导EL-4淋巴瘤细胞凋亡及细胞周期进程适应性反应的时间效应

刘淑春1，赵文举2，孙丽光1，李鹏武1吕 喆1，龚守良 1   

  1. 1.吉林大学公共卫生学院 卫生部放射生物学重点实验室，吉林 长春 130021；2.解放军第208医院妇产科，吉林 长春 130062
  • 收稿日期:2007-10-09 修回日期:1900-01-01 出版日期:2008-07-28 发布日期:2008-07-28
  • 通讯作者: 龚守良

Time effect of adaptive response of apoptosis and cell cycle progression induced by low-dose ionizing radiation in EL-4 lymphoma cells in vitro

LIU Shu-chun1,ZHAO Wen-ju2,SUN Li-guang1,LI Peng-wu1,LU Zhe1,GONG Shou-liang 1   

  1. 1.Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China；2.Department of Obstetrics and Genecology,No.208 Hospital of PLA,Changchun 130062,China)
  • Received:2007-10-09 Revised:1900-01-01 Online:2008-07-28 Published:2008-07-28
  • Contact: GONG Shou-liang

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摘要： 目的：研究低剂量辐射诱导EL-4淋巴瘤细胞凋亡及细胞周期进程适应性反应的时间效应，以揭示低剂量辐射生物效应及其诱导适应性反应的可能机制。方法：实验分D2组（攻击剂量）、D1（诱导剂量）+ D2组和假照组。用X射线照射离体EL-4淋巴瘤细胞，其D1为75 mGy（剂量率12.5 mGy•min-1），D2为1.5 Gy（剂量率287 mGy•min-1），D1和D2间隔为3~60 h。通过流式细胞仪检测其细胞凋亡和细胞周期进程的变化。结果：当D1和D2间隔为3~24 h时，D1+D2组细胞凋亡百分数明显低于D2组（P<0.05或P<0.01），G0/G1期细胞百分数不同程度低于D2组，而S期细胞百分数明显高于D2组（P<0.05）。结论：75 mGy（12.5 mGy•min-1）照射后3~24 h，进行1.5 Gy（287 mGy•min-1）照射，可诱导体外EL-4淋巴瘤细胞凋亡和细胞周期进程的适应性反应。

关键词: 淋巴瘤细胞, 细胞凋亡, 细胞周期, 适应性反应

Abstract: To study the time effect of adaptive response of apoptosis and cell cycle progression induced by low-dose ionizing radiation in EL-4 lymphoma cells in vitro to reveal the possible mechanism of biological effect and adaptive response induced by low-dose radiation. Methods The experiment was divided into D2 (challenging dose) group,D1 (inductive dose)+D2 group and sham-irradiation group. EL-4 lymphoma cells were irradiated with D1 (75 mGy,12.5 mGy•min-1) and D2 (1.5 Gy,287 mGy•min-1),the interval time between D1 and D2 were 3-60 h. The cell percentages of apoptosis and each cell cycle phase were measured with flow cytometry. Results When the time intervals between D1 and D2 were 3-24 h,the percentages of apoptotic cells in D1+D2 group were significantly lower than those in D2 group (P<0.05 or P<0.01); meantime,the percentages of G0/G1 phase cells decreased in varying degrees,and the percentage of S phase cells increased significantly (P<0.05). Conclusion The adaptive response of apoptosis and cell cycle progression in EL-4 lymphoma cells in vitro could be induced by irradiation with 75 mGy (12.5 mGy•min-1) at 3-24 h before 1.5 Gy (287 mGy•min-1) exposure.

Key words: lymphoma cells, apoptosis, cell cycle, adaptive response

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