A total of 60 healthy adult female Wistar rats were selected and divided into normal group， model group and experimental group， there were 20 rats in each group. The rats in model group and experimental group were used to establish the CFS models by multiple stress， and abdominal manipulation intervention was performed in experiment group after model establishment， while the rats in normal group received no treatment.The behavior effect indicators were used to verify the models； the urtramicromorphology of hippocampal neurons of the rats was observed by electron microscope. The positive expression rates of FK506 binding protein （FKBPs），glucocorticoid receptor （GR） and N-methyl-D-aspartate receptor （NMDAR） were detected by immunohistochemical method， and the serum levels of adrenocorticotropic hormone （ACTH），cortisol（CORT），corticotropin releasing hormone （CRH） and glucocorticoid （GC） of the rats were detected by ELISA method.

The behavior effect indicator examination results showed the time for immobility of the rats in model group and experiment group before abdominal manipulation was longer than that in normal group（P<0.05），and the time for exhaustive swimming and the speeds of horizontal movement of the mice in model group and experiment group were lower than those in normal group（P<0.05）；compared with model group， the time for immobility of the rats in experiment group was significantly shortened（P<0.05），the exhaustive swimming time was significantly prolonged（P<0.05），and the horizontal movement speed was significantly increased（P<0.05）.Compared wtih normal group，shrinkage of hippocampal neurons， some invaginated neurons and apoptotic bodies were observed in model group under electron microscope；compared with model group， the hippocampal neurons in experiment group were significantly alleviated. Compared with normal group， the positive expression rates of FKBPs， GR and NMDAR in the hippocampus tissue of the rats in model group and experiment group were significantly decreased（P<0.05）； compared with model group， the positive expression rates of FKBPs， GR and NMDAR in the hippocampus tissue of the rats in experiment group were significantly increased（P<0.05）. Compared with normal group， the serum levels of ACTH， CORT， CRH and GC of the rats in model group were significantly increased（P<0.05）； compared with model group， the serum levels of ACTH， CORT， CRH and GC of the rats in experiment group were significantly decreased（P<0.05）.

Abdominal manipulation can promote the remodeling of damaged hippocampal neurons caused by stress response， reduce the levels of ACTH， CORT and CRH， and maintain the negative feedback balance of hippocampus-HPA axis through regulating FKBPs-GR-NMDAR pathway.