青春期前双酚A暴露对雌性大鼠卵巢组织中mRNA分子表达谱的影响

doi:10.13481/j.1671-587X.20210609

Abstract

Abstract: Objective

To study the effect of bisphenol A （BPA） on the mRNA molecule expression profile in ovarian tissue of the prepubertal female rats， and to explore the potential effect of BPA on the structure and function of ovarian tissue.

Methods

A total of 32 female SD rats were randomly divided into control group and low，middle，and high doses of BPA groups，and there were 8 rats in each group.The rats in low，middle，and high doses of BPA groups were given 50，100，and 200 mg·kg^－1 BPA，and the rats in control group were given ethanol-corn oil（1∶100）. HE staining was used to observe the morphology of ovarian tissue of the rats in various groups. RNA sequencing （RNA-seq） and bioinformatic analyses were used to predict the differential expression genes and their biological functions between high dose of BPA group and control group. The mRNA expression levels of Hapln3， Nr4a1， Arc， Ccl2， Ccn5， Egr2，and Rab33a in ovarian tissue of the rats in various groups were detected by Real-time fluorescence quantitative PCR （RT-qPCR）method.

Results

The HE staining results showed that compared with control group， there were no obvious abnormalities in ovarian tissue of the rats in low and middle doses of BPA groups. The number of primordial follicles， pre sinus follicles and sinus follicles in ovarian tissue of the rats in high dose of BPA group were decreased， the number of atretic follicles was increased， and the cystic follicles were appeared.The RNA-seq detection results showed that there were 14 differential expression genes in ovarian tissue of the rats in control group and high dose of BPA group. The bioinformatics analysis results showed that 14 mRNA molecules were involved in a variety of biological processes such as reproduction， metabolism and apoptosis， as well as p53， MAPK， PI3K-Akt，and other signal pathways. Compared with control group， the expression levels of Hapln3， Nr4a1， Arc， Ccl2， Ccn5，and Egr2 mRNA in ovarian tissue of the rats in high dose of BPA group were decreased （P<0.05），and the expression level of Rab33a mRNA was increased （P<0.05）.

Conclusion

BPA changes the mRNA molecule expression profile in ovarian tissue of the prepubertal female rats， which may potentially influence the structure and function of ovarian tissue.

Key words: bisphenol a, endocrine disrupting chemicals, ovarian tissue, mRNA, rat，SD, prepubertal

CLC Number:

R994.6

Xueying ZHANG,Lianwen ZHENG,Yinggang ZOU,Zhenhuan ZHOU. Effect of bisphenol A exposure on mRNA molecule expression profile in ovarian tissue of prepubertal female rats[J].Journal of Jilin University(Medicine Edition), 2021, 47(6): 1407-1414.

Figures/Tables 7

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References 29

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Group	Body weight
Group	PND28	PND32	PND36	PND41
Control	100.43±0.75	121.80±1.11	141.34±1.69	156.11±3.20
BPA
Low dose	103.43±2.01	126.88±2.91	146.98±3.22	157.03±4.04
Middle dose	101.79±1.36	122.61±1.79	143.44±2.27	157.85±3.08
High dose	98.95±1.20	121.79±1.37	144.01±1.77	157.16±2.84

Group	Ovarian weight （m/mg）	Ovarian coefficient（η/%）
Control	20.480±0.759	0.130±0.004
BPA
Low dose	19.350±0.974	0.120±0.006
Middle dose	18.310±1.333	0.120±0.008
High dose	14.180±1.282 ^*	0.090±0.007 ^*

Group	Hapln3	Nr4a1	Arc	Ccl2	Ccn5	Egr2	Rab33a
Control	1.014 7±0.117 3	1.014 7±0.117 3	1.014 7±0.117 3	1.014 7±0.117 3	1.014 7±0.117 3	1.014 7±0.117 3	1.014 7±0.117 3
High dose of BPA	0.078 5±0.014 4^*	0.078 5±0.014 4^*	0.078 5±0.014 4^*	0.078 5±0.014 4^*	0.078 5±0.014 4^*	0.078 5±0.014 4^*	0.078 5±0.014 4^*

KEGG Pathway Classification	Related Genes
Cytokine-cytokine receptor interaction	Inhba, Ccl2
Apoptosis - multiple species	Pmaip1
Aldosterone synthesis and secretion	Nr4a1
NOD-like receptor signaling pathway	Ccl2
p53 signaling pathway	Pmaip1
TGF-beta signaling pathway	Inhba
TNF signaling pathway	Ccl2
Signaling pathways regulating pluripotency of stem cells	Inhba
Apoptosis	Pmaip1
Chemokine signaling pathway	Ccl2
MAPK signaling pathway	Nr4a1
PI3K-Akt signaling pathway	Nr4a1

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Effect of bisphenol A exposure on mRNA molecule expression profile in ovarian tissue of prepubertal female rats

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