Journal of Jilin University(Medicine Edition) ›› 2022, Vol. 48 ›› Issue (3): 638-647.doi: 10.13481/j.1671-587X.20220312
• Research in basic medicine • Previous Articles
Xiaochen HUANG1,Hao LI2,Baohua WANG1(),Kai LI2,3()
Received:
2021-09-22
Online:
2022-05-28
Published:
2022-06-21
Contact:
Baohua WANG,Kai LI
E-mail:wbhzyxx@163.com;likai@jlu.edu.cn
CLC Number:
Xiaochen HUANG,Hao LI,Baohua WANG,Kai LI. Protective effect of lidocaine on PC12 cells in Parkinson’s disease model and its mechanism[J].Journal of Jilin University(Medicine Edition), 2022, 48(3): 638-647.
Tab.2
Expression levels of Wnt1 and caspase 3 proteins in PC12 cells in various groups after treated with lidocaine for 6 h"
Group | Wnt1 | caspase 3 |
---|---|---|
Control | 0.360±0.010 | 0.537±0.007 |
MPTP | 0.035±0.005* | 0.815±0.045* |
MPTP+0.200 g·L-1 lidocaine | 1.105±0.045*△ | 0.755±0.005* |
MPTP+0.600 g·L-1 lidocaine | 1.575±0.055*△# | 0.660±0.020△ |
MPTP+1.000 g·L-1 lidocaine | 0.630±0.008*△#○ | 0.436±0.02△#○ |
F | 337.0 | 47.21 |
P | <0.01 | <0.01 |
Tab.4
Levels of IL-1β,IL-6,and TNF-α in supernatant of PC12 cells in various groups [n=3,x±s, ρB/(ng·L-1)]"
Group | IL-1β | IL-6 | TNF-α |
---|---|---|---|
Control | 1.173±0.011 | 98.732±2.576 | 3.135±0.437 |
MPTP | 1.935±0.083* | 172.561±1.053* | 24.421±1.578* |
MPTP+lidocaine | 1.203±0.033△ | 1005.020±4.722△ | 6.081±1.387△ |
MPTP+lidocaine+DKK1 | 2.122±0.003*# | 157.634±0.726*# | 33.186±0.240*△# |
MPTP+DKK1 | 2.834±0.011*△#○ | 161.525±4.615*# | 29.030±0.530*# |
F | 92.38 | 292.3 | 1916 |
P | <0.01 | <0.01 | <0.01 |
Tab.5
Expression levels of Wnt1,β-catenin,GSK-3β,p-GSK-3β,and caspase 3 proteins in PC12 cells in various groups"
Group | Wnt1 | β-catenin | GSK-3β | p-GSK-3β | caspase 3 |
---|---|---|---|---|---|
Control | 0.961±0.008 | 0.975±0.005 | 0.517±0.004 | 0.545±0.005 | 0.544±0.006 |
MPTP | 0.563±0.011* | 0.408±0.004* | 0.568±0.008* | 0.772±0.014* | 0.580±0.010* |
MPTP+lidocaine | 0.609±0.011*△ | 0.476±0.010*△ | 0.503±0.004*△ | 0.419±0.004*△ | 0.288±0.003*△ |
MPTP+lidocaine+DKK1 | 0.399±0.008*△# | 0.340±0.010*△# | 0.729±0.010*△# | 0.519±0.005△# | 0.351±0.021*△# |
MPTP+DKK1 | 0.427±0.095*△# | 0.490±0.010*△# | 0.721±0.005*△# | 0.575±0.015*△# | 0.705±0.015*△#○ |
F | 690.2 | 2 325 | 342.5 | 190.6 | 209.5 |
P | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
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