甲型流感病毒PR8感染小鼠早期记忆T淋巴细胞的改变及其意义

doi:10.13481/j.1671-587X.20250614

Abstract

Abstract:

Objective To discuss the changes of early memory T lymphocytes （T_M） in the mice infected with influenza A virus （H1N1） PR8， and to clarify its significance. Methods A total of 32 C57BL/6 mice were randomly divided into control group and influenza virus （PR8） group， with 16 mice in each group. The mice in control group were intranasally administered 30 μL of sterile phosphate buffered saline （PBS）， and the mice in PR8 group were intranasally administered 2 LD₅₀ PR8 virus solution. The mice were sacrificed on the 10 th day， and bronchoalveolar lavage fluid （BALF）， lung tissue， and lymphnodes were collected for subsequent experiments. HE staining was used to observe the pathomorphology of lung tissue in the mice in two groups； hemagglutination assay， real-time fluorescence quantitative PCR （RT-qPCR） method， and immunofluorescence method were used to detect the viral infection in lung tissue of the mice； flow cytometry was used to detect the percentages of T lymphocytes， effector T lymphocytes， central memory T lymphocytes （T_CM）， effector memory T lymphocytes （T_EM）， and nuclear protein（NP）/pdymerase acidic protein（PA）-specific T_EM lymphocytes in BALF， lung tissue， and lymphnodes of the mice in two groups. Results Compared with control group， the lung tissue of the mice in PR8 group showed significant pathological damage， and the lung tissue virus titer and NP protein expression level were significantly increased （P<0.01）. Compared with control group， the percentages of CD4⁺ T lymphocytes， CD8⁺ T lymphocytes， effector CD4⁺ T lymphocytes， effector CD8⁺ T lymphocytes， CD4⁺ T_EM lymphocytes， and CD8⁺ T_EM lymphocytes in BALF and lung tissue of the mice in PR8 group were significantly increased （P<0.01）. Compared with control group， the positive percentages of CD4⁺ T_CM， CD8⁺ T_CM， CD4⁺ T_EM， CD8⁺ T_EM， and NP/PA-specific CD4⁺ T_EM， CD8⁺ T_EM lymphocytes in BALF and lung tissue of the mice in PR8 group were significantly increased （P<0.05 or P<0.01）， and the percentage of CD8+ T_CM lymphocytes in the lymph nodes was significantly increased （P<0.05）. Conclusion Influenza virus infection can induce the proliferation and differentiation of T lymphocytes into effector T lymphocytes， leading to the early establishment of virus-specific T_M lymphocytes targeting the conserved NP/PA epitopes in the respiratory mucosa.

Key words: Influenza virus, Memory T lymphocyte, Central memory T lymphocyte, Effector memory T lymphocyte, Conserved epitope

CLC Number:

R392

Kun XU,Jing TIAN. Changes of early memory T lymphocytes in mice infected with influenza A virus PR8 and its significance[J].Journal of Jilin University(Medicine Edition), 2025, 51(6): 1584-1594.

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Changes of early memory T lymphocytes in mice infected with influenza A virus PR8 and its significance

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