Journal of Jilin University Medicine Edition
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DENG Wang,ZHAO Yan,HE Jing,WANG Dao-xin
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Abstract:
To investigate the role of insulin in the expression of sodium channel β subunit (β-ENaC) in alveolar epithelial A549 cells,and
to clarify the possible signaling pathway on regulating β-ENaC by insulin. Methods The alveolar epithelial A549 cells were
cultured in vitro and divided into control group,insulin group (A549 cells were incubated with 2,20, and 200 mU•L-1 insulin for
30,60,and 120 min),and LY294002 group,respectively.The expression levels of mRNA and protein of β-ENaC and p-Akt protein were
determined by RT-PCR and Western blotting methods.Results Compared with control group,the expression levels of β-ENaC mRNA and
protein were elevated significantly after treated with 2,20,and 200 mU•L-1 insulin in a concentration-dependent manner
(P<0.05).Compared with control group,the expression levels of p-AKt protein were elevated significantly after treated with
2,20,and 200 mU?L-1 insulin(P<0.05) in a concentration-dependent manner.Compared with control group,the expression levels of β-
ENaC mRNA and protein were elevated significantly after treated with insulin for 30,60,and 120 min in a time-dependent manner
(P<0.05).Compared with insulin group,the expression levels of β-ENaC mRNA and protein and p-Akt protein in LY294002 group were
decreased(P<0.05).Conclusion Activation of PI3K/Akt signaling pathway can increase the expression levels of β-ENaC mRNA and
protein,which is beneficial for clearance of pulmonary edema fluid.
Key words: epithelial sodium channel &beta, subunit;phosphatidylinositol 3-kinase;protein kinase B;signaling pathway;insulin
CLC Number:
DENG Wang,ZHAO Yan,HE Jing,WANG Dao-xin. Regulation of insulin in expression of alveolar epithelial sodium channel β subunit by PI3K signaling pathway[J].Journal of Jilin University Medicine Edition, 2014, 40(02): 233-237.
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URL: http://xuebao.jlu.edu.cn/yxb/EN/10.13481/j.1671-587x.20140204
http://xuebao.jlu.edu.cn/yxb/EN/Y2014/V40/I02/233
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