Abstract:

Abstract:Objective
 To investigate the influence of salvianolic acid B on the expressions of TGF-β1/Smad signalingpathway related proteins in human lung fibroblasts,and to explore the mechanism of the inhibitoryeffect of Sal B on TGF-β1-induced lung fibroblast activation.Methods The human embryonic lungfibroblasts (MRC-5) were cultured in vitro and randomly divided into control group ( the cells were cultured with DMEM without TGF-β1 or Sal B),Sal B group (the cells were cultured with 10  μmol•L^-1 Sal B),TGF-β1 group (the cells were cultured with 10 μg•L^-1 TGF-β1),and TGF-β1(10 μg•L^-1) + SalB (10  μmol•L^-1) group.The protein levels of p-Smad2,p-Smad3,TβRⅠ, and Smad7 in the fibroblastsin various groups were detected by Western blotting method. Results Compared with control group,the expression levels of p-Smad2,p-Smad3, and TβRⅠ proteins in TGF-β1 group were significantly increased (P<0.05),and the expression level of Smad7 protein was decreased (P<0.05).Compared with control group,the expression levels of p-Smad2,p-Smad3,TβRⅠ, and Smad7 proteins in lung fibroblasts in Sal B group had no significant change (P>0.05).Compared with TGF-β1 group,the expression levels of p-Smad2,p-Smad3, and TβRⅠ in TGF-β1+ Sal B group were descended (P<0.05),and the expression level of Smad7 was increased (P<0.05).Conclusion Sal B could suppress the TGF-β/Smad signaling pathway in lung fibroblasts and  to inhibit the  TGF-β1-induced lung fibroblast activation.

Key words: salvianolic acid B, lung fibroblast, transforming growth factor-&beta, 1；Smad proteins