Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (02): 235-239.doi: 10.13481/j.1671-587x.20150206

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Effects of hydrogen sulfide on expressions of MT1-MMP and TIMP-2 proteins and myocardial fibrosis in myocardium tissue of diabetic rats and their mechanisms

CHU Chun1, ZENG Ou2, LUO Jian2, LI Fang2, XIAO Ting2, ZHOU Songbai2, YANG Jun2   

  1. 1. Department of Pharmacy, Second Affiliated Hospital, South China University, Hengyang 421001, China;
    2. Department of Cardiology, First Affiliated Hospital, South China University, Hengyang 421001, China
  • Received:2014-08-07 Online:2015-03-28 Published:2015-04-04

Abstract:

Objective To explore the effects of hydrogen sulfide (H2S) on the myocardial fibrosis and the expressions of membrane-type 1 matrixmetallo-proteinase (MT1-MMP) and tissue inhibitor of metalloproteinase-2(TIMP-2) in the myocardium tissue of the rats with diabetes mellitus(DM),and to clarify the effect of H2S on the development of the myocardial fibrosis and its mechanism.Methods 52 rats were randomly divided into control group,DM group,DM+H2S group and H2S group (n=13).The rat models were induced by intraperitoneal injection of streptozotocin (STZ). The rats in control group(n=12) and DM group(n=9) were injected with saline after modeling and the rats in DM+H2S group(n=9) and H2S group(n=10) were administrated with 100 mg·kg-1 NaHS.After 8 weeks,the pathomorphology of myocardium tissue of the rats in various groups were analyzed by VG staining;the expression levels of type Ⅲ collagen,MT1-MMP and TIMP-2 proteins in the myocardium tissue of the rats were analyzed by Western blotting method.Results Compared with control group,disorgnised array in the myocardial cells,collagen deposition and myocardial fibrosis of the rats were found in DM group;the expression levels of type Ⅲ collagen,MT1-MMP and TIMP-2 proteins in myocardium tissue of the rats were significantly increased (P<0.05).Compared with DM group,the myocardial fibrosis was improved of the rats in DM+H2S group,and the expression levels of type Ⅲ collagen,MT1-MMP and TIMP-2 proteins in the myocardium tissue of the rats in DM+H2S group were significantly decreased (P<0.05).Conclusion H2S could improve the myocardial fibrosis and decrease the expression of type Ⅲ collage protein in diabetic rats and its mechanism may be related to the down-regulation of MT1-MMP and the improvement of the imbalance of MT1-MMP/TIMP-2.

Key words: hydrogen sulfide, membrane-type 1 matrixmetallo-proteinase, tissue inhibitor of metalloproteinase-2, diabetes mellitus, myocardial fibrosis

CLC Number: 

  • R587.1