肝祖细胞移植对四氯化碳诱导急性肝衰竭小鼠模型肝损伤的修复作用

doi:10.13481/j.1671-587x.20150306

Abstract

Abstract:

Objective To obseve the rapair effect of hepatic progenitor cells (HPCs) transplantation on the liver of mouse models of acute liver failure induced with different concentrations of carbon tetrachloride (CCl₄), and to construct one ideal mouse model of acute liver failure for evaluation on the efficacy of HPCs transplantation. Methods 96 ICR mice were randomly divided into nine groups: blank control group, negative control groups (0.1% CCl₄ group, 0.5% CCl₄ group, 2.0% CCl₄ group and 10.0% CCl₄ group) and experimental groups (0.1% CCl₄ + HPCs group, 0.5% CCl₄ + HPCs group, 2.0% CCl₄ + HPCs group and 10.0% CCl₄ + HPCs group).Normal saline was given to the mice in blank control group by intragastric administration, and the mice in negative control groups and experimental groups were administrated with different concentrations of CCl₄.One day later, the mice in experimental groups were injected with HPCs from spleen, and the mice in negative control groups were given the same dose of normal saline.14 d after the injection of HPCs, the survival rate, liver index, the activities of serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST), the histopathological changes, the distribution of exogenous hepatocytes and the expression levels of cytokeratin 18(CK18) and albumin (ALB) were detected. Results Compared with blank control group, the survival rates, liver indexes and the activities of serum ALT and AST in 0.1% CCl₄ group and 0.5% CCl₄ group had no significant changes(P>0.05);the effective treatment of HPCs transplantation was not observed because of self-repairing.Compared with 2.0% CCl₄ model group, the activities of serum ALT and AST and liver index in 2.0% CCl₄ + HPCs group were significantly decreased(P<0.01), and the survival rate was increased(P<0.01);the liver cell regeneration and a large number of exogenous hepatocytes were found by pathological detection in 2% CCl₄ + HPCs group;the therapy of HPCs transplantation was effective.All mice died in 2 d in 10.0% CCl₄ group and 10.0% CCl₄ + HPCs group, and the therapeutic efficacy of HPCs was not observed. Conclusion HPCs transplantation can increase the survival rate of the model mice of acute liver failure, improve the activities of ALT, and AST and repair the hepatic injury;HPCs transplantation treatment is effective on the mouse model of 2.0% CCl₄-induced acute liver failure.

Key words: carbon tetrachloride, acute liver failure, hepatic progenitor cells, transplantation

CLC Number:

R575.3

ZHAO Li, HUANG Daochao, GONG Mengjia, LI Yasha, BI Yang. Repair effect of hepatic progenitor cell transplantation on acute liver failure mouse model induced by carbon tetrachloride[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 464-469.

References

[1] Filippi C, Dhawan A.Current status of human hepatocyte transplantation and its potential for Wilson's disease[J].Ann N Y AcadSci, 2014, 1315:50-55.

[2] Stock P. Age-dependent hepatocyte transplantation for functional liver tissue reconstitution[J].Methods Mol Biol, 2014, 1213:41-50.

[3] 杨永平, 周霖, 马伟, 等.肝细胞移植治疗重型肝炎临床观察[J].解放军医学杂志, 2007, 32(1):12-15.

[4] 赵晓彪, 明玉玲, 陈平.肝切除术后肝脏再生的研究进展[J].解放军医学杂志, 2014, 39(1):70-74.

[5] PietrosiG, Vizzini GB, Gruttadauria S, et al.Clinical applications of hepatocyte transplantation[J].WordJ Gastroenterol, 2009, 15(17):2074-2077.

[6] Dhawan A, Mitry RR, Hughes RD.Hepatocyte transplantation for liver-based metabolic disorders[J].J Inherit Metab Dis, 2006, 29(23):431-435.

[7] Pareja E, Cortes M, Bonora A, et al.New alternatives to the treatment of acute liver failure[J].Transplant Proc, 2010, 42(8):2959-2961.

[8] Darwiche H, Petersen BE.Biology of the adult hepatic progenitor cell: "ghosts in the machine"[J].Prog Mol Biol Transl Sci, 2010, 97:229-249.

[9] Clouston AD, Jonsson JR, Powell EE.Hepatic progenitor cell-mediated regeneration and fibrosis: chicken or egg?[J].Hepatology, 2009, 49(5):1424-1426.

[10] Jamshidzadeh A, Fereidooni F, Salehi Z, et al.Hepatoprotective activity of Gundeliatourenfortii[J].J Ethnopharmacol, 2005, 101(1-3):233-237.

[11] Schmelzer E, Zhang L, Bruce A, et al.Human hepatic stem cells from fetal and postnatal donors[J].J Exp Med, 2007, 204(8):1973-1987.

[12] Tosh D.Hepatic progenitor cells[J].Cell Tissue Res, 2010, 342(2):131-137.

[13] Bin WT, Ma LM, Xu Q, et al.Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action[J].World J Gastroenterol, 2012, 18(4):309-322.

[14] Khan AA, Parveen N, Mahaboob VS, et al.Treatment of Crigler-Najjar Syndrome type 1 by hepatic progenitor celltransplantation: a simple procedure for management of hyperbilirubinemia[J].Transplant Proc, 2008, 40(4):1148-1150.

[15] Ghasemi M, Azarnia M, Jamali M.Protective effects of Ephedra pachyclada extract on mouse models of carbon tetrachloride- induced chronic and acute liver failure[J].Tissue Cell, 2014, 46(1):78-85.

[16] 阎向东, 童英, 郑珊, 等.急性四氯化碳致大、小鼠肝损害程度的比较[J].中国卫生检验杂志, 2005, 15(8):989-990.

[17] 刘长海, 华春秀, 归改霞, 等.和厚朴酚对小鼠四氯化碳肝损伤的保护作用[J].郑州大学学报:医学版, 2013, 48(4):470-473.

[18] 童方念, 罗超, 罗丹, 等.山茶酚对四氯化碳致小鼠急性肝损伤的保护作用[J].西安交通大学学报:医学版, 2014, 35(6):816-819.

[19] Katoonizadeh A, Poustchi H, Malekzadeh R.Hepatic progenitor cells in liver regeneration: current advances and clinical perspectives[J].Liver Int, 2014, 10(24):1464-1472.

[20] Delladetsima J, Alexandrou P, Giaslakiotis K, et al.Hepatic progenitor cells in chronic hepatitis C: a phenomenon of older age and advanced liver disease[J].Virchows Arch, 2010, 457(4):457-466.

[21] Yang F, Li X, Wang LK, et al.Inhibitions of NF-kappaB and TNF-alpha result in differential effects in rats with acute on chronic liver failure induced by d-Gal and LPS[J].Inflammation, 2014, 37(3):848-857.

Repair effect of hepatic progenitor cell transplantation on acute liver failure mouse model induced by carbon tetrachloride

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	MA Yalan, ZHANG Xin, LI Dandan, ZHOU He, FANG Shujia, XU Ying, ZHENG Lianwen. Influence of high-purity human menopausal gonadotropin added in process of antagonist program COH in IVF-ET pregnancy outcome in patients with normal ovarian function reserve [J]. Journal of Jilin University(Medicine Edition), 2018, 44(05): 1036-1040.
[2]	WANG Mingrui, ZHAI Xiuyu, WANG Gang, ZHANG Wenlan, GAO Baoshan, ZHOU Honglan, FU Yaowen. Effects of CYP3A5 genetic polymorphisms on metabolism of tacrolimus in patients after kidney transplantation [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 537-542.
[3]	CHEN Biao, ZHANG Rui, ZHANG Wenjuan, YUE Lei, FAN Xuhui, LIU Jilun, LIU Yaoqiang, CUI Yi, QU Pengfei, YANG Wei. Repair effect of dental pulp stem cells on facial nerve injury in rabbits and its mechanism [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 504-509.
[4]	ZHANG Lipeng, RONG Chunshu, GU Yiming, FU Yaowen, GAO Baoshan. Dynamicfeatures of B lymphocyte reconstruction in peripheral blood of patients with immune tolerance induced with combined transplantation of kidney and bone marrow [J]. Journal of Jilin University Medicine Edition, 2018, 44(02): 332-338.
[5]	PENG Yinyin, CHEN Liping, LIU Lin, CHEN Jianbin, CHEN Yajuan, HAN Xiaoli, XIAO Qing. Rituximab-induced interstitial lung disease:A case report and literature review [J]. Journal of Jilin University Medicine Edition, 2017, 43(06): 1260-1264.
[6]	YI Xue, WU Pengyu, YANG Shuliang, HAN Yan, YANG Xuehui, ZHANG Shubo, XU Tao, GAO Yizhe, GAO Muhuo, LI Zhanqing. Influence of sulforaphane preconditioning in myocardial cold ischemia-reperfusion injury through PI3K/Akt signaling pathway in rats [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 918-922.
[7]	LONG Yi, LUO Jingfang, HU Minyu, CHEN Jihua, ZHU Liufeng, WEI Wei, QIAO Nan, YANG Lina. Protective effect of ellagic acid on acute liver injury induced by CCl₄ in mice and its mechanism [J]. Journal of Jilin University Medicine Edition, 2017, 43(03): 572-576.
[8]	ZHENG Wen, CHEN Baofeng, LI Jie, ZHOU Yingying. Application value of high frequence ultrasound in reinjection of facial autologous fat granule injection transplantation [J]. Journal of Jilin University Medicine Edition, 2017, 43(01): 170-175.
[9]	WANG Zhiguo, LIU Xueming, TONG Shengquan, SHI Zhequn, RAO Li. Therapeutic effect of mesenchymal stem cell transplantation on pulmonary hypertension in rats [J]. Journal of Jilin University Medicine Edition, 2017, 43(01): 21-25.
[10]	CHEN Yang, E Changyong, GUAN Lianyue, LIU Wentao, WANG Lina, ZHANG Xuewen. Establishment of liver regeneration model of cirrhosis mice induced by CCl₄ after partial hepatectomy and its evaluation [J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1243-1248.
[11]	LU Yan, QIN Xiuhong, MA Xiang, LU Zhili. Application of bandage contact lens in pterygium excision combined with conjunctival transplantation in older adults and evaluation on its efficacy [J]. Journal of Jilin University Medicine Edition, 2016, 42(05): 1005-1009.
[12]	QIAO Sha, HUANG Yinghui, WANG Shibao, SUN Yanxia, LU Zhenxia. Influence of different culture methods in DNA methylation status of breast cancer MDA-MB-231 cells [J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 271-276.
[13]	HAN Xueli, LIU Dong, ZHANG Xiujuan, ZHOU Ying. Evaluation effect of autologous bone marrow stem cell transplantation combined with percutaneous endovascular angioplasty in treatment of diabetic foot [J]. Journal of Jilin University Medicine Edition, 2016, 42(01): 125-129.
[14]	ZHAO Minxue, LI Bowen, WANG Desheng, CAO Hong. Role of miR-122 in bone marrow derived stem cell therapy for acute liver injuryin rats [J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1150-1153.
[15]	ZHAO Yan, XIAO Yulong, ZUO Yuan, WANG Xiliang, HUO Hongjun, JIANG Jianming, YAN Huibo. Repair effect of neural stem cells transplantation combined with erythropoietin on spinal cord injury in rats [J]. Journal of Jilin University Medicine Edition, 2015, 41(02): 333-337.