鹿茸多肽对人骨髓间充质干细胞BMP-2和Runx2表达的影响

doi:10.13481/j.1671-587x.20150311

Abstract

Abstract:

Objective To investigate the influence of velvet antler polypeptides (VAP) in the expressions of bone morphogenetic protein-2 (BMP-2) and runt-related transcription factor 2 (Runx2) gene and protein in human bone marrow mesenchymalstem cells (hBMSCs), and to clarify the mechanism of VAP in bone synthesis and bone differentiation direction. Methods The cultured hBMSCs were divided into control group and 5, 10, 15, 20 g ·L^-1 VAP groups.By detecting the alkaline phosphatase (ALP) activities in hBMSCs in various groups, the optimal concentration of the drug in vitro was selected;CCK-8 assay was used to detect the proliferation of hBMSCs in control group and 10 g·L^-1 VAP group;the BMP-2 and Runx2 gene and protein expressions in control group and 10 g·L^-1 VAP group were determined with fluorescence quantitative PCR and Western blotting method. Results The ALP activities in VAP groups were higher than that in control group, and the ALP activity in 10 g ·L^-1 VAP group was the highest (P<0.01).The CCK-8 assay Results showed that the proliferation rate of the hBMSCs in 10 g ·L^-1 VAP group was higher than that in control group (P<0.05), the VAP-induced proliferation of cultured hBMSCs was the highest at the 9th day, then went into the plateau and began to decline;the quantitative PCR and Western blotting Results showed that the expression levels BMP-2 and Runx2 gene and protein in the hBMSCs in 10 g ·L^-1 VAP group were significantly higher than those in control group, and the differences were statistically significant(P<0.05). Conclusion VAP can improve the ALP activity in hBMSCs and promote the proliferation and differentiation of hBMSCs, and increase the expressions of BMP-2 gene and its Runx2 gene and protein, which may be one of the molecular regulation mechanisms of VAP for bone formation and bone metabolism of hBMSCs.

Key words: velvet antler polypeptides, human bone marrow mesenchymal stem cells, bone morphogenetic protein-2, runt-related transcription factor 2

CLC Number:

R961

ZHANG Hongchang, ZHANG Ying, LIU Mingxin, PAN Zhi, LYU Guangfu, CHEN Gang, JIANG Hongyuan, GUO Junqi. Influence of velvet antler polypeptides in expressions of BMP-2 and Runx2 in human bone marrow mesenchymal stem cells[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 491-495.

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Influence of velvet antler polypeptides in expressions of BMP-2 and Runx2 in human bone marrow mesenchymal stem cells

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