Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (03): 506-509.doi: 10.13481/j.1671-587x.20150314

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Effects of different physical and chemical factors on purity of recombinant human fibroblast growth factor 21

WANG Changwen1, LIU Min1, LIU Lei2, JIANG Yong2, ZHANG Lei2, LI Yan2, WANG Huiyan2   

  1. 1. Department of Nutrition and Food Hygiene, School of Public Health, Jilin Medical College, Jilin132013, China;
    2. Department of Biotechnology, School of Medical Laboratory, Jilin Medical College, Jilin 132013, China
  • Received:2014-09-02 Published:2015-08-01

Abstract:

Objective To study the stabilities of recombinant human fibroblast growth factor 21 (rhFGF21) deposited at different conditions, and to elucidate the influence of temperature, buffer solutions and repeated freeze thawing in the stability of rhFGF21. Methods rhFGF21 was deposited at different conditions such as temperatures (-20℃, 4℃, 25℃), buffer solutions (acetate sodium acetate buffer pH 4.0 and pH 5.0, citric acid-sodium citrate buffer pH 5.0 and pH 6.0, disodium hydrogen phosphate and sodium dihydrogen phosphate buffer pH 6.0, pH 6.5and pH 7.0, Tris - HCl buffer pH 7.5 and pH8.0) and repeated freeze thawing, respectively.The purity of rhFGF21 was determined by HPLC, and the appearance was also observed. Results All properties of rhFGF21 were not altered during the observation period at -20℃ and 4℃;the purity of rhFGF21 deposied for 14 d was reduced to (15.20±0.14)% compared with 0 d(100%±0.00%) at 25℃(P<0.05).The acetate sodium acetate buffer pH 4.0 and pH 5.0 and citric acid sodium citrate buffer pH 5.0 and pH 6.0 were turbidity under the condition of 25℃ for 7 d;the disodium hydrogen phosphate and sodium dihydrogen phosphate buffer pH 6.5 and pH 7.0, and Tris-HCl buffer pH 7.5 and pH 8.0 were clear transparent under the condition of 25℃ for 7 d. The purity of rhFGF21 had significant change after repeated with freeze thawing (P<0.05). Conclusion rhFGF21 is stable when deposited at low temperature and pH 6.5-8.0 neutral conditions.High temperature, acidic and basic conditions are harmful for the stability of rhFGF21.It is profit for rhFGF21 stability in sodium dihydrogen phosphate buffer.There is impact on rhFGF21 stability after freeze thrawing repeatedly.

Key words: recombinant human fibroblast growth factor 21, purity, stability, physical and chemical factors

CLC Number: 

  • Q78