源于人躯干皮肤真皮的成纤维样细胞的间充质干细胞分化潜能

doi:10.13481/j.1671-587x.20160211

Abstract

Abstract:

Objective: To study the differentiation capacity of the fibroblast-like cells isolated from human skin dermis into mesenchymal stem cells, and to explore the feasibility to use these cells as alternative cell source of autologus bone marrow mesenchymal stem cells (BMSCs) for regeneration of tissue injuries and defects.Methods: Full thickness skin samples were obtained from the abdomen of surgical patients, then digested with dispase and collagenase Ⅰ subsequently.Thereafter, the digested cells were collected and cultured, followed by suspension with serum free medium containing N2, B27, basic fibroblast growth factor(bFGF), and epidermal growth factor(EGF).The skin dermis derived spheroids(SDDSs) were collected and monolayer cultured in serum-containing medium.Finally, the cells were characterized by immunofluorescence staining and differentiation assays.Results: The dermis derived cells proliferated and formed SDDSs in the suspension of serum-free medium.After monolayer cultivation in serum-containing medium, the cells from spheroids were successfully expanded to large number.The cells expressed mesenchymal stem cells markers CD90, CD105 and vimentin.Under osteogenic, chondrogenic and adipogenic differentiation conditions, these cells were differentiated into the alizarin red, safranin O, and oil red O staining positive cells, displayed similar differentiation traits with BMSCs.However, safranin O staining was weaker in the dermis derived cells than BMSCs.Conclusion: A kind of fibroblast-like cells exist in human skin dermis, and have osteocytic, chondrogenic and adipogenic differentiation potentials, demonstrating that these cells will be utilized as a novel cell source for repairing the tissue injury and defect in clinic.

Key words: skin, dermis, mesenchymal cells, chondrocytes, bone cells

CLC Number:

R318

LYU Shuang, XU Jinying, FENG Wei, ZHANG Zechuan, LI Meiying, BI Bo, SUN Dongjie, LI Yulin, CHI Guangfan. Potential to differentiation of human dermis-derived fibroblast-like cells into mesenchymal stem cells in vitro[J].Journal of Jilin University Medicine Edition, 2016, 42(02): 245-249.

References

[1] Usategui A,del Rey MJ,Pablos JL. Fibroblast abnormalities in the pathogenesis of systemic sclerosis[J].Expert Rev Clin Immunol,2011,7(4):491-498.

[2] 马晓飞,朱明,张艳,等.真皮成纤维细胞多向分化潜能的研究进展[J].组织工程与重建外科杂志,2011,7(6):347-349.

[3] Lorenz K,Sicker M,Schmelzer E,et al.Multilineage differentiation potential of human dermal skin-derived fibroblasts[J].Exp Dermatol,2008,17(11):925-932.

[4] Han X,Han J,Ding F,et al.Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells[J].Cell Res,2011,21(10):1509-1512.

[5] Chen FG,Zhang WJ,Bi D,et al.Clonal analysis of nestin(-) vimentin(+) multipotent fibroblasts isolated from human dermis[J].J Cell Sci,2007,120(Pt 16):2875-2883.

[6] 晁洋,倪华,兰青艳,等.旋转发生器培养扩增人皮肤成纤维细胞[J].实用口腔医学杂志,2011,27(3):356-360.

[7] 王悦,计阿丹,肖永红.17β-雌二醇对小鼠肺成纤维细胞周期的影响[J].郑州大学学报:医学版,2014,49(4):530-532.

[8] Nayak S,Dey S,Kundu SC.Skin equivalent tissue-engineered construct:co-cultured fibroblasts/keratinocytes on 3D matrices of sericin hope cocoons[J].PLoS One,2013,8(9):e74779.

[9] Okita K,Ichisaka T,Yamanaka S.Generation of germline-competent induced pluripotent stem cells[J].Nature,2007,448(7151):313-317.

[10] Yu J,Vodyanik MA,Smuga-Otto K,et al.Induced pluripotent stem cell lines derived from human somatic cells[J].Science,2007,318(5858):1917-1920.

[11] Li X,Zuo X,Jing J,et al.Small-molecule-driven direct reprogramming of mouse fibroblasts into functional neurons[J].Cell Stem Cell,2015,17(2):195-203.

[12] Hu W,Qiu B,Guan W,et al.Direct conversion of normal and Alzheimer's disease human fibroblasts into neuronal cells by small molecules[J].Cell Stem Cell,2015,17(2):204-212.

[13] Brendel C,Kuklick L,Hartmann O,et al.Distinct gene expression profile of human mesenchymal stem cells in comparison to skin fibroblasts employing cDNA microarray analysis of 9600 genes[J].Gene Expr,2005,12(4-6):245-257.

[14] Yamanaka S.Induction of pluripotent stem cells from mouse fibroblasts by four transcription factors[J].Cell Prolif,2008,41(suppl 1):51-56.

[15] Toma JG,McKenzie IA,Bagli D,et al.Isolation and characterization of multipotent skin-derived precursors from human skin[J].Stem Cells,2005,23(6):727-737.

[16] Kuroda Y,Kitada M,Wakao S,et al.Unique multipotent cells in adult human mesenchymal cell populations[J].Proc Natl Acad Sci USA,2010,107(19):8639-8643.

[17] Tsuchiyama K,Wakao S,Kuroda Y,et al.Functional melanocytes are readily reprogrammable from multilineage-differentiating stress-enduring (muse) cells,distinct stem cells in human fibroblasts[J].J Invest Dermatol,2013,133(10):2425-2435.

Potential to differentiation of human dermis-derived fibroblast-like cells into mesenchymal stem cells in vitro

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