慢病毒介导靶向P27RF-Rho基因沉默对肝癌细胞侵袭性的影响

doi:10.13481/j.1671-587x.20160214

Abstract

Abstract:

Objective: To investigate the silencing of P27RF-Rho gene with lenvirus targeting mediated technique, and to clarify its influence in the invasion of liver cancer cells.Methods: The P27RF-Rho RNAi lentivirus was constructed.The liver cancer BEL7402 cells were infected with lentivirus.The experiment was divided into P27RF Rho-siRNA group, scramble-siRNA group and BEL7402 group. The effect of silencing P27RF-Rho gene and the expression levels of hepatocellular carcinoma (HCC) associated proteins RhoA, RhoC, VEGF, P53 and PTEN were detected;the activities of matrix metalloproteinase (MMPs)associated with tumor invasion were analyzed by Gelatin zymography; the variation of transfer ability and invasion abilities were compared by Wound healing assay experiment and Transwell experiment.Results: The Western blotting results showed the expression levels of P27RF-Rho, RhoA, RhoC, and VEGF proteins in the BEL7402 cells in experiment group were significantly lower than those in two control groups(P<0.05), and the expression levels of P53 and PTEN were higher than those in two control groups(P<0.05).The results of Gelatin zymography showed the activities of MMPs in experiment group were significantly lower than those in two control groups(P<0.01);Wound healing assay showed that the migration ability of the BEL7402 cells in experiment group was significantly inhibited (P<0.01);the number of cells passed through the Transwell Chambers in experiment group was significantly less than those in two control groups (P<0.01).Conclusion: Silenceing P27RF-Rho can weaken the invasion ability and migration ability of human HCC BEL7402 cells.

Key words: P27RF-Rho, lentivirus, liver cancer cells, invasion and migration

CLC Number:

R735.7

MA Qiang, XIE Shuli, WANG Guangyi, XING Guangyuan, YANG Yaoqun, LYU Guoyue. Influence of lentiviral-mediated P27RF-Rho gene silence in invasion of liver cancer cells[J].Journal of Jilin University Medicine Edition, 2016, 42(02): 260-265.

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Influence of lentiviral-mediated P27RF-Rho gene silence in invasion of liver cancer cells

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