不同浓度熊果酸对破骨细胞增殖分化的作用及其意义

doi:10.13481/j.1671-587x.20170205

Abstract

Abstract: Objective: To investigate the effects of ursolic acid (UA) on the proliferation and differentiation of osteoclasts (OC), and to explore its role in orthodontic force-induced root resorption and its relationship with OC. Methods: The mononuclear/macrophage cells RAW264.7 were induced to the OC.Tacrolimus acid phosphatase (TRAP) staining and bone resorption lacunae observation were used to identify the induction. CCK-8 method was used to select the appropriate concentration of UA for RAW264.7 cell-free biotoxicity and to observe its effect on the proliferation and differentiation of RAW264.7.In experimental groups, UA with gradient concentrations (1.0,2.5,5.0,10.0,20.0and 40.0 μmol·L^-1)were added.UA was not added in control group. Results: The TRAP staining and bone resorption lacunae observation showed that after the RAW264.7 cells were induced for 5 d, the TRAP staining positive cells were found; the resorption lacunae were rounded,and oval, etc,the bottom wall was coarser,and the boundary was clear,which indicated that the RAW264.7 cells were successfully differentiated into the osteoclasts.The CCK-8 detection results showed that high concentration of UA (> 10.0 μmol·L^-1) significantly inhibited the proliferation of OC;the appropriate concentration of UA (5.0 μmol·L^-1) was in the biological safety concentration range and could inhibit the OC proliferation; low concentration of UA (<2.5 μmol·L^-1) had no effect. Conclusion: RANKL can induce the differentiation and maturation of RAW264.7 cells. UA is correlated with the proliferation and differentiation of OC; UA has inhibitory effect on OC at the appropriate concentration (5.0 μmol·L^-1) in a time-dependent manner.

Key words: osteoclasts, ursolic acid, root resorption, RAW264.7 cells

CLC Number:

R329.2

WANG Sihan, JIANG Huan, YU Hang, LIU Shaowei, LI Menghong, WANG Liuyi, YANG Junxing, HU Min. Effects of ursolic acid at different concentrations on proliferation and differentiation of osteoclasts and their significances[J].Journal of Jilin University Medicine Edition, 2017, 43(02): 236-240.

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Effects of ursolic acid at different concentrations on proliferation and differentiation of osteoclasts and their significances

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