PLGA涂层处理的nHA-Mg多孔复合材料对兔颌骨缺损的修复作用

doi:10.13481/j.1671-587x.20170213

Abstract

Abstract: Objective: To observe the repair effect of nano hydroxyapatite/magnesium(nHA-Mg)porous composite material modified by poly lactic acid/glycolic acid copolymer(PLGA) on the jaw bone defect of rabbits,and to elucidate the mechanisms preliminary. Methods: The mandibular defect models of 10 mm×5 mm×1 mm in the 18rabbits were established.Nine rabbits were selected and implanted with nHA-Mg composite materials modified by PLGA in the left as experimental group and the right as blank control group;the other nine were implanted with nHA-Mg in the left as positive control group and the right as blank control group.The rabbits were sacrificed at 4, 8, 12 weeks (respectively 3 of experimental group and positive control group every time)and the mandibular defect areas were intercepted and observed by imaging and histological examination;the sizes of the newborn trabecula area and residual materials in experimental group and positive control group were compared. Results: Compared with positive control group and blank control group, the percentage of newborn trabecula area of the rabbits in experimental group was increased (P<0.05),and there was no statistic difference between positive control group and blank control group (P>0.05).The imaging results showed that the new bone formation can be observed in experimental group and better than positive control group and blank control group.The paraffin section results indicated that there were visible vascular tissue and newborn trabecula,the osteoblasts gathered around the bone trabecula; a lot of pits were located in the bone trabecula, and the pits contained osteocytes in experimental group.As the prolongation of time,thickened newborn trabecula, dense arrangement and trend of converting to lamellar bone were observed in experimental group.The hard tissue section results showed that the remaining amount of materials in experimental group was more than that in positive control group. Conclusion: nHA-Mg porous composite materials modified by PLGA can effectively reduce the rate of degradation in the body, promote osteogenesis and guide the bone regeneration.

Key words: jaw bone defect, nano-hydroxyapatite, magnesium, poly lactic acid/glycolic acid

CLC Number:

R782.2

CHE Hongze, CHE Yanhai, LU Qing, CHEN Nannan, CHEN Fei, JIN Quan, MA Ning. Repair effect of nHA-Mg porous composite materials modified by PLGA on jaw bone defect of rabbits[J].Journal of Jilin University Medicine Edition, 2017, 43(02): 276-280.

References

[1] 卢晓蕾.颌骨缺损修复方法的进展[J].黑龙江医药科学,2012,35(2):89-90.
[2] Crane GM,Ishaug SL,Mikos AG.Bone tissue engineering[J].Nat Med,1995,1(12):1322-1324.
[3] Venkatesan J,Kim SK.Nano-hydroxyapatite composite biomaterials for bone tissue engineering-a review[J].Biomed Nanotechnol,2014,10(10):3124-3140.
[4] 傅婷,谷子芽,李颖,等.纳米羟基磷灰石复合物在骨组织工程中的应用与挑战[J].医学研究杂志,2016,45(2):153-155.
[5] 朱阳,李道伟,方滕姣子,等.载EPO腺病毒的PLGA纳米纤维支架在体内促进骨缺损修复作用[J].吉林大学学报:医学版,2015,41(4):711-715.
[6] 袁景,甄平.骨组织工程支架的研究进展[J].医学综述,2015,21(12):2126-2130.
[7] 杨再清,雷云坤,孟增东.纳米羟基磷灰石在骨科中的临床应用及作用机制[J].中国组织工程研究,2012,16(51):9629-9634.
[8] Tsao YP,Neiva R,Al-Shammari K,et al.Factors influencing treatment outcomes in mandibular Class Ⅱ furcation defects[J].J Periodontol,2006,77(4):641-646.
[9] 王健,马翔宇,冯亚非,等.镁离子对成骨细胞活力和分化的促进作用及其机制研究[J].现代生物医学进展,2015,15(15):2836-2839.
[10] 杨俊宇,徐永清,何晓清.镁及镁合金材料作为骨科内植物研究进展[J].中国修复重建外科杂志,2016,30(12):1562-1566.
[11] Liu YJ,Yang ZY,Tan LL,et al.An animal experimental study of porous magnesium scaffold degradation and osteogenesis[J].Braz J Med Biol Res,2014,47(8):715-720.
[12] 何畔,仲琦,卢建忠,等.nHA/Mg/壳聚糖膜复合材料的制备及耐腐蚀性研究[J].口腔医学,2015,35(6):433-437.
[13] Ewald A,Hösel D,Patel S,et al.Silver-doped calcium phosphate cements with antimicrobial activity[J].Acta Biomater,2011,7(11):4064-4070.
[14] Chen G, Ushida T,Tateishi T. Scaffold design for tissue engineering[J]. Macromol Biosci, 2002,2(2):67-77.
[15] O'Brien FJ. Biomaterials & scaffolds for tissue engineering[J]. Material Today, 2011,14(3):88-95.
[16] Webster TJ, Ergun C, Doremus RH,et al.Enhanced functions of osteoblasts on nanophase ceramics[J]. Biomaterials,2000,21(17):1803-1810.
[17] Musso CG.Magnesium metabolism in health and disease[J].Int Urol Nephrop,2009,41(2):357-362.
[18] 王树峰,王程越,姚玉胜,等.AZ31镁合金修复兔下颌骨缺损的实验研究[J].口腔医学研究,2013(1):33-36.
[19] Liang D,Yang M,Guo B,et al.Zinc inhibits H₂O₂-induced MC3T3-E1 cells apoptosis via MAPK and PI3K/AKT pathways[J].Biol Trace Elem Res,2012,148(3):420-429.
[20] Rangaswami H,Schwappacher R,Tran T,et al.Protein kinase G and focal adhesion kinase converge on Src/Akt/β-catenin signaling module in osteoblast mechanotransduction[J].J Biol Chem,2012,287(25):21509-21519.
[21] 赵常利,张绍翔,何慈晖,等.生物医用镁合金表面PLGA涂层研究[J].功能材料,2008,39(6):987-989.
[22] 康文慧.nHA/Mg复合材料经CS及PLGA表面修饰后对MG-63细胞BMP-2蛋白表达的影响[D].长春:吉林大学,2015.

Repair effect of nHA-Mg porous composite materials modified by PLGA on jaw bone defect of rabbits

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