BCG感染牛肺泡巨噬细胞的转录组测序和分析

doi:10.13481/j.1671-587x.20190103

Journal of Jilin University(Medicine Edition) ›› 2019, Vol. 45 ›› Issue (01): 12-16.doi: 10.13481/j.1671-587x.20190103

Transcriptomesequencing and analysis of bovine alveolar macrophages infected with BCG

XU Jinrui, LYU Cuiping, YANG Yi, ZHOU Yanbing, LUO Jia, WANG Yujiong

Key Laboratory of Protection and Utilization of Special Biological Resources in Western China, Ministry of Education, Ningxia University, Yinchuan 750021, China

Received:2018-05-24 Published:2019-01-28
Contact: 国家自然科学基金资助课题（31572494，31760733）；宁夏回族自治区科技厅自然科学基金资助课题（2018AAC03019）；宁夏回族自治区重点研发计划项目资助课题（东西部合作，2017BN04） E-mail:wyj@nxu.edu.cn
Supported by:
This work was supported by Fundaçaõ de Amparo à Pesquisa do Estado de Saõ Paulo (FAPESP) (Grant 2009/52237-9 to WV, fellowship 2014/23951-3 to LMSM).

Abstract

Abstract: Objective: To reveal the differentially expressed genes and long non-coding RNAs (lncRNAs) by sequencing the transcriptome of bovine alveolar macrophages (BAM) infected with Bacillus Calmette-Guérin (BCG) and analyzing their bioinformations, and to provide theoretical foundation for the research on immune regulation mechanism of anti-infection of Mycobacterium tuberculosis of macrophages.Methods: The BAM were collected by pulmonary lavage and centrifugation and cultured and divided into infected group and uninfected group.After infection for 12 h in infected group,the expression profiles of mRNA and lncRNA in infected group and uninfected group were detected by RNA-Seq, and bioinformatics analysis was carried out.Results: compared with uninfected group, there were 119 differentially expressed lncRNAs and 1111 differentially expressed mRNA in infected group (P<0.05). Gene Ontology functional enrichment analysis showed that the most significant enrichment was immune response (GO:0006955, P<0.05), including 125 genes, in which 63 were up-regulated and 62 were down-regulated, and the expressions of proinflammatory factors interleukin-1 (IL-6), interleukin-7 (IL-7), and interleukin-23A (IL-23A) were up-regulated. Cis target gene prediction and KEGG pathway analysis showed that the differentially expressed lncRNAs were involved in the regulation of transforming growth factor-β (TGF-β) signaling pathway and ATP-binding cassette transporter (ABC transporter) signaling pathway (P<0.05).Conclusion: The host cells are stimulated to produce a strong immune response after the BAM are infected by BCG and results in the changes of lncRNA and mRNA expression profiles.

Key words: Bacillus Calmette-Guérin, bovine alveolar macrophage, transcriptome sequencing, long non-coding RNA

CLC Number:

Q939.91

XU Jinrui, LYU Cuiping, YANG Yi, ZHOU Yanbing, LUO Jia, WANG Yujiong. Transcriptomesequencing and analysis of bovine alveolar macrophages infected with BCG[J].Journal of Jilin University(Medicine Edition), 2019, 45(01): 12-16.

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Transcriptomesequencing and analysis of bovine alveolar macrophages infected with BCG

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