坐骨神经脉冲射频对慢性坐骨神经压迫损伤模型大鼠脊髓背角胶质细胞活化水平的影响及其镇痛作用

doi:10.13481/j.1671-587x.20190109

Abstract

Abstract: Objective: To observe the effect of sciatic nerve pulsed radiofrequency(PRF) on the activation levels of microglia cells and astrocytes in spinal dorsal horn in the chronic constriction injury(CCI) rat models, and to explore the relationships between the analgesic mechanism of sciatic nerve PRF and the activation levels of microglia cells and astrocytes in the spinal dorsal horn.Methods: Forty male SD rats were randomly divided into CCI sham-operation+RPF sham group(SS group), CCI sham-operation+RPF group(SP group), CCI+RPF sham group(CS group), CCI+RPF group(CP group)(n=10). PRF was applied on the sciatic nerve on the 4th day after CCI operation for 120 s, with a maximum temperature of 42℃.The mechanical withdrawal thresholds (MWT) and thermal paw withdrawal latencies (TWL) were measured to evaluate mechanical hyperalgesia and thermal hyperalgesia 1 d before operation (D₀) and 1,3,5,7 d after operation (D₁, D₃, D₅, D₇). Western blotting method was used to detect the protein expression levels of ionized calcium binding adapter molecule 1(Iba-1) and glial fibrillary acidic protein (GFAP) in the ipsilateral spinal dorsal horn of L3-5 after pain behavioral test(D₇).Results: Compared with SS group, the rats in CS group after CCI had the significant behavioral changes, such as hallux valgus, lameness, toe bending, and foot raising during walking; MWT and TWL were decreased significantly(P<0.01); the expression levels of Iba-1 and GFAP proteins in the ipsilateral spinal dorsal horn were increased significantly(P<0.05). Compared with CS group, the behavioral changes of the rats in CP group (D₅, D₇) such as hallux valgus, lameness, toe bending, and foot raising during walking were alleviated significantly; MWT and TWL were increased significantly(P<0.01); the Iba-1 protein expression level in spinal dorsal horn was down-regulated significantly (P<0.05) and the GFAP protein expression level did not change significantly (P>0.05).Conclusion: PRF on sciatic nerve can relieve the neuropathic pain (NP) of the CCI rat models; PRF on sciatic nerve can inhibit activation of the microglia cells in the spinal dorsal horn. The analgesic mechanism of PRF on sciatic nerve may be associated with the inhibition of the activation of microglia cells in the spinal dorsal horn.

Key words: pulsed radiofrequency, chronic constriction injury model, neuropathic pain, astrocytes, microglia cells, spinal dorsal horn

CLC Number:

R-332

JIANG Ren, FENG Zhiying, LI Ping, LI Hong, LI Shuangyue. Effect of sciatic nervepulsed radiofrequency glial activation levels in spinal dorsal horn in chronic constriction injury rat models and its analgesia effect[J].Journal of Jilin University(Medicine Edition), 2019, 45(01): 45-50.

References

[1] HAANPAA M,ATTAL N,BACKONJA M, et al. NeuPSIG guidelines on neuropathic pain assessment[J].Pain,2011,152(1):14-27.
[2] LOGGIA M L,CHONDE D B,AKEJU O, et al. Evidence for brain glial activation in chronic pain patients[J]. Brain,2015,138(Pt 3):604-615.
[3] KIM K, JO D,KIM E. Pulsed radiofrequency to the dorsal root ganglion in acute herpes zoster and postherpetic neuralgia[J]. Pain Physician,2017,20(3):E411-E418.
[4] YOON Y M,HAN S R,LEE S J, et al. The efficacy of pulsed radiofrequency treatment of cervical radicular pain patients[J]. Korean J Spine,2014,11(3):109-112.
[5] SHANTHANNA H, CHAN P,MCCHESNEY J, et al. Pulsed radiofrequency treatment of the lumbar dorsal root ganglion in patients with chronic lumbar radicular pain:a randomized, placebo-controlled pilot study[J]. J Pain Res,2014,7:47-55.
[6] CHANG M C. Efficacy of pulsed radiofrequency stimulation in patients with peripheral neuropathic pain:A narrative review[J]. Pain Physician, 2018,21(3):E225-E234.
[7] SLUIJTER M E. Pulsed radiofrequency[J]. Anesthesiology,2005,103(6):1313.
[8] CHOI Y H,CHANG D J,HWANG W S, et al. Ultrasonography-guided pulsed radiofrequency of sciatic nerve for the treatment of complex regional pain syndrome type Ⅱ[J]. Saudi J Anaesth,2017,11(1):83-85.
[9] PICELLI A,LOBBA D,VENDRAMIN P, et al. A retrospective case series of ultrasound-guided suprascapular nerve pulsed radiofrequency treatment for hemiplegic shoulder pain in patients with chronic stroke[J]. J Pain Res,2018,11:1115-1120.
[10] ABD-EISAYED A, JACKSON M, Plovanich E. Pulsed radiofrequency ablation for treating sural neuralgia[J]. Ochsner J,2018,18(1):88-90.
[11] JIA Z, REN H, LI Q, et al. Pulsed radiofrequency reduced neuropathic pain behavior in rats associated with upregulation of GDNF expression[J]. Pain Physician,2016,19(2):49-58.
[12] LI D Y,MENG L,JI N, et al. Effect of pulsed radiofrequency on rat sciatic nerve chronic constriction injury:a preliminary study[J]. Chin Med J,2015,128(4):540-544.
[13] LIU Y,FENG Y,ZHANG T. Pulsed radiofrequency treatment enhances dorsal root ganglion expression of hyperpolarization-activated cyclic nucleotide-gated channels in a rat model of neuropathic pain[J]. J Mol Neurosci,2015,57(1):97-105.
[14] WU B,NI J,ZHANG C, et al. Changes in spinal cord met-enkephalin levels and mechanical threshold values of pain after pulsed radio frequency in a spared nerve injury rat model[J]. Neurol Res,2012,34(4):408-414.
[15] YEH C C, WU Z F, CHEN J C, et al. Association between extracellular signal-regulated kinase expression and the anti-allodynic effect in rats with spared nerve injury by applying immediate pulsed radiofrequency[J]. BMC Anesthesiol,2015,15:92.
[16] AUSTIN P J,MOALEM-TAYLOR G. The neuro-immune balance in neuropathic pain:involvement of inflammatory immune cells, immune-like glial cells and cytokines[J]. J Neuroimmunol,2010,229(1/2):26-50.
[17] SUN J S, YANG Y J, ZHANG Y Z, et al. Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats[J]. Mol Med Rep,2015,12(2):2677-2682.
[18] PARK H W, AHN S H, SON J Y, et al. Pulsed radiofrequency application reduced mechanical hypersensitivity and microglial expression in neuropathic pain model[J]. Pain Med,2012,13(9):1227-1234.
[19] VALLEJO R,TILLEY D M,VOGEL L, et al. The role of glia and the immune system in the development and maintenance of neuropathic pain[J]. Pain Pract,2010,10(3):167-184.
[20] LIU R, XU X, XU Y, et al. Pulsed radiofrequency on dorsal root ganglion relieved neuropathic pain associated with downregulation of the spinal interferon regulatory factor 8, microglia, p38MAPK expression in a CCI rat model[J]. Pain Physician,2018,21(4):E307-E322.
[21] AKAGI T, MATSUMURA Y,YASUI M, et al. Interferon regulatory factor 8 expressed in microglia contributes to tactile allodynia induced by repeated cold stress in rodents[J]. J Pharmacol Sci,2014,126(2):172-176.
[22] LIN M L, LIN W T, HUANG R Y, et al. Pulsed radiofrequency inhibited activation of spinal mitogen-activated protein kinases and ameliorated early neuropathic pain in rats[J]. Eur J Pain,2014,18(5):659-670.
[23] LEE J B,BYUN J H,CHOI I S, et al. The effect of pulsed radiofrequency applied to the peripheral nerve in chronic constriction injury rat model[J]. Ann Rehabil Med,2015,39(5):667-675.
[24] 李坚,李刚,郭卫东,等.TGN-020对大鼠脊髓损伤后继发性水肿和星形胶质细胞增生的影响[J]. 西安交通大学学报:医学版,2018,39(4):685-690,718.

Effect of sciatic nervepulsed radiofrequency glial activation levels in spinal dorsal horn in chronic constriction injury rat models and its analgesia effect

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Metrics

Comments

Recommended 0

[1]	LIU Linna, CHEN Jieyu, LYU Fangyi, HUA Kouzhen, CAI Damin, YIN Guoli, WANG Junjuan. Effect of fingolimod on proliferation and migration of neural stem cells and astrocytes and its protective mechanism against spinal cord injury in mice [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1218-1222.
[2]	MA Li-na, YU Hong-mei,ZHAO Wen-jing, LUO Tao, CHEN Sen, NOMOTO Hiroshi, FURUKAWA Shoei. Construction and identification of astrocyte linesexpressing NGF, BDNF, and NT3 steadily [J]. J4, 2005, 31(2): 227-230.
[3]	MA Li-na, ZHAO Wen-jing, LUO Tao, NOMOTO Hiroshi，FURUKAWA Shoei. Establishment of culture system of conditionalizationfor astrocytes of cerebral cortex in vitro [J]. J4, 2004, 30(5): 727-729.
[4]	LI Xian-hui, CHENG Yi, LIU Ya-wen , WANG Gang, LI Ji-sheng. Expression of nerve growth factor gene in astrocytescultured in 1,25-(OH)₂D₃ defined medium [J]. J4, 2004, 30(3): 398-400.