神经生长因子对脑梗死大鼠脑组织中生长分化因子15表达水平的影响

doi:10.13481/j.1671-587x.20190317

Abstract

Abstract: Objective:To investigate the effect of nerve growth factor (NGF) on the expression level of growth differentiation factor-15(GDF-15) in the brain tissue of the rats with cerebral infarction, and to elucidate the mechanism of NGF in the rats with cerebral infarction. Methods:The middle cerebral artery occlusion (MCAO) models were established by the Longa's method. A total of 54 SD rats were randomly divided into sham operation group, model group and NGF group, and there were 18 rats in each group.The rats in NGF group were given NGF (50 μg·kg^-1) by intraperitoneal injection,and equal volume of normal saline was given to the rats in sham operation (the artery was isolated without ligation) group and model group. The neurological function scores of the rats in various groups were measured 1, 3 and 7 d after operation; HE staining was used to observe the pathomorphology of nerves in brain tissue and immunohistochemical staining was used to detect the number of GDF-15 positive cells; the expression levels of GDF-15 in brain tissue of the rats in various groups were detected by ELISA method. Results:The results of HE staining showed that the degrees nerve cell necrosis, interstitial edema and glial cell proliferation were relatively low in NGF group 7 d after operation. Compared with sham operation group, the neurological function scores, the number of GDF-15 positive cells,and the expression levels of GDF-15 in brain tissue of the rats in model group and NGF group were significantly increased (P<0.05). Compared with model group, the neurological function scores of the rats in NGF group were significantly decreased 3 and 7 d after operation (P<0.05), and the number of GDF-15 positive cells and the expression levels of GDF-15 in brain tissue of the rats in NGF group were significantly increased at different time points after operation (P<0.05). Conclusion:NGF can protect the brain nerve by up-regulating the expression level of GDF-15 in brain tissue and improving the nerve function.

Key words: cerebral infarction, nerve growth factor, growth differentiation factor 15, neurological function, rats,SD

CLC Number:

R743.33

JIAO Guangmei, SHAN Hailei, DOU Zhijie, ZHAO Liang, ZHANG Xiaoxuan, KANG Lingling, MA Zheng, YANG Ning. Effect of nerve growth factor on expression level of growth differentiation factor-15 in brain tissue of rats with cerebral infarction[J].Journal of Jilin University(Medicine Edition), 2019, 45(03): 572-576.

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Effect of nerve growth factor on expression level of growth differentiation factor-15 in brain tissue of rats with cerebral infarction

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