大鼠心脏TRPV1受体激活对心血管活动的抑制作用

doi:10.13481/j.1671-587x.20190503

Abstract

Abstract: Objective:To investigate the cardiovascular reflex induced by the activation of cardiac transient receptor potential vanillic acid receptor 1 (TRPV1), the central location of reflex and its peripheral neurotransmitters of the rats, and to elucidate the central and peripheral neural mechanisms of cardiovascular reflex mediated by TRPV1 receptor. Methods:A total of 60 healthy male SD rats were randomly divided into control group, ido-RTX group, atropine group, immunofluorescence group and nerve tract tracking group;there were 12 rats in each group. Pericardial intubation was performed in the rats in various groups after anesthesia. The changes of heart rates and blood pressures of the rats in control group,ido-RTX group and atropine group were observed by arterial intubation.The tissue samples were collected 1 h after intropericardial injection of capsaicin in imunofluorescence group,and the co-expression of c-Fos positive neurons induced by capsaicin and cholinesterase positive neurons in the medulla oblongata was observed by immunofluorescence technigue. Intraopericardial injected carbonyl cyanine fluorescent dye (Dil) was used as retrograde tracer in nerve tract group,and the distribution of Dil positive cells in the medulla oblongata was observed by fluorescence microscope 1 week later. Results:The blood pressure and heart rate of the rats in control group were decreased significantly after intrapericardial injection of capsaicin of the rats in control group (P<0.05).Compared with control group,the blood pressures and heart rates of the rats in ido-RTX group and atropine group had no significant changes after intrapericardial injection of capsaicin following intrapericardial injection of TRPV1 antagonist ido-RTX and intravenous injection of M receptor antagonist atropine(P>0.05); after intrapericardial injection of capsaicin, the number of c-Fos positive neurons in nucleus tractus solitarii,dorsal motor nucleus of vagus and nucleus ambiguous of medulla oblongata was increased significantly (P<0.05);most of these neurons were cholinesterase positive neurons; after intrapericardial injection of Dil, a large number of red fluorescent labeled neurons and nerve endings appeared in the nucleus tractus solitarii,dorsal motor nucleus of vagus and nucleus ambiguous. Conclusion:Intrapericardial injection of capsaicin can selectively activate the TRPV1 receptors in the heart, increase the excitability of cholinergic neurons in the cardiovascular center of the medulla oblongata, increase the peripheral release of acetylcholine, and produce an inhibitory effect on the cardiovascular activity by activating the M receptor.

Key words: cardiac injury receptor, transient receptor potential vanilloid 1 receptor, meddulla oblongata cardiaovascular center, cardiovascular reflex, M receptor

CLC Number:

R338.22

LIU Xiaohua, HAN Man, ZHANG Qi. Inhibitory effect of activation of cardiac TRPV1 receptors on cardiovascular activity in rats[J].Journal of Jilin University(Medicine Edition), 2019, 45(05): 992-996.

References

[1] FOREMAN RD, GARRETT KM, BLAIR RW. Mechanisms of cardiac pain[J]. Compr Physiol, 2015, 5(2):929-960.
[2] GIBBINS I. Functional organization of autonomic neural pathways[J]. Organogenesis, 2013, 9(3):169-175.
[3] TOSCHI-DIASE,RONDON M U P B,COGLIATI C, et al. Contribution of autonomic reflexes to the hyperadrenergic state in heart failure[J]. Front Neurosci, 2017, 11:162.
[4] ZAHNER M R, LI D P, CHEN S R,et al. Cardiac vanilloid receptor 1-expressing afferent nerves and their role in the cardiogenic sympathetic reflex in rats[J]. J Physiol(Lond), 2003, 551(Pt2):515-523.
[5] CINCA J,RODRÍGUEZ-SINOVAS A. Cardiovascular reflex responses induced by epicardial chemoreceptor stimulation[J]. Cardiovasc Res, 2000, 45(1):163-171.
[6] LIU X H, ZHANG Q, HAN M,et al. Intrapericardial capsaicin and bradykinin induce different cardiac-somatic and cardiovascular reflexes in rats[J]. Auton Neurosci, 2016, 198:28-32.
[7] CRISAFULLI A. The Impact of cardiovascular diseases on cardiovascular regulation during exercise in humans:Studies on metaboreflex activation elicited by the post-exercise muscle ischemia method[J]. Curr Cardiol Rev, 2017, 13(4):293-300.
[8] CATERINA M J, SCHUMACHER M A, TOMINAGA M,et al. The capsaicin receptor:a heat-activated ion channel in the pain pathway[J]. Nature, 1997, 389(6653):816-824.
[9] QUARTU M, SERRA M P, BOI M,et al. TRPV1 receptor in the human trigeminal ganglion and spinal nucleus:immunohistochemical localization and comparison with the neuropeptides CGRP and SP[J]. J Anat, 2016, 229(6):755-767.
[10] AGHAZADEH TABRIZI M, BARALDI P G, BARALDI S,et al. Medicinal chemistry, pharmacology, and clinical implications of TRPV1 receptor antagonists[J]. Med Res Rev, 2017, 37(4):936-983
[11] HERMES S M, ANDRESEN M C, AICHER S A. Localization of TRPV1 and P2X3 in unmyelinated and myelinated vagal afferents in the rat[J]. J Chem Neuroanat, 2016, 72:1-7.
[12] MOHAMMED M, MADDEN C J, ANDRESEN M C,et al. Activation of TRPV1 in nucleus tractus solitarius reduces brown adipose tissue thermogenesis, arterial pressure, and heart rate.[J]. Am J Physiol Regul Integr Comp Physiol, 2018, 315(1):R134-R143.
[13] HE B, LU Z B, HE W B,et al. Autonomic modulation by electrical stimulation of the parasympathetic nervous system:An emerging intervention for cardiovascular diseases[J]. Cardiovasc Ther, 2016, 34(3):167-171.
[14] BRAUNWALD E. The war against heart failure:the Lancet lecture[J]. Lancet, 2015, 385(9970):812-824.
[15] GAO YF, SONG JX, CHEN H, et al TRPV1 activation is involved in the cardioprotection of remote limb ischemic postconditioning in ischemia-reperfusion injury rats[J]. Biochem Biophys Res Commun, 2015, 463(4):1034-1039.
[16] ICHIGE M H, SANTOS C R, JORDÃO C P,et al. Exercise training preserves vagal preganglionic neurones and restores parasympathetic tonus in heart failure[J]. J Physiol(Lond), 2016, 594(21):6241-6254.
[17] ZHANG J H, WU Z P, FAN ZP,et al. Pericardial application as a new route for implanting stem-cell cardiospheres to treat myocardial infarction[J]. J Physiol(Lond), 2018, 596(11):2037-2054.
[18] 冯丹阳,孙强,陈莉娜,等.Ghrelin对糖尿病大鼠心肌动作电位和瞬时外向钾电流的影响[J].西安交通大学学报:医学版,2017,38(5):661-664.
[19] LI J, ZHANG M M, TU K,et al. The excitatory synaptic transmission of the nucleus of solitary tract was potentiated by chronic myocardial infarction in rats[J]. PLoS One, 2015, 10(3):e0118827.
[20] HUA F, HARRISON T, QIN C,et al. C-Fos expression in rat brain stem and spinal cord in response to activation of cardiac ischemia-sensitive afferent neurons and electrostimulatory modulation[J]. Am J Physiol Heart Circ Physiol, 2004, 287(6):H2728-H2738.
[21] LIU X H, HAN M, ZHU J X,et al. Metabotropic glutamate subtype 7 and 8 receptors oppositely modulate cardiac nociception in the rat nucleus tractus solitaris[J]. Neuroscience, 2012, 220:322-329.

Inhibitory effect of activation of cardiac TRPV1 receptors on cardiovascular activity in rats

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Metrics

Comments

Recommended 0

[1]	LIU Xiao-hua,HAN Man,DU Jian-qing. Role of glutamate receptor subtypes in cardiac nociception in nucleus tractus solitarius in rats [J]. Journal of Jilin University Medicine Edition, 2014, 40(03): 508-512.
[2]	HAN Man, SUN Na, LIU Xiao-hua, DU Jian-qing. Modulation of NMDA receptor to rat cardiacsomatic motor reflex induced by intrapericardial bradykinin in spinal cord [J]. J4, 2012, 38(5): 832-835.