17β-雌二醇对失血性休克家兔肠系膜微循环的改善作用及其机制

doi:10.13481/j.1671-587x.20190602

Abstract

Abstract: Objective: To observe the improvement effect of 17β-estradiol on the intestinal mucosal mesenteric microcirculation after hemorrhagic shock (HS) in the rabbits,and to explore the relevant mechanism. Methods: Thirty-two rabbits were randomly divided into model group,saline treatment group,anisodamine treatment group and 17β-estradiol prevention group,and there were 8 rabbits in each group.The HS model was made by Chaudry method,and the changes of intestinal mucosal microcirculation were recorded;the pathomorphology of intestinal mucosa of the rabbits in various groups were observed under microscope and the Chiu's score was used to evaluate.the positive expression rates of interleukin-6 (IL-6) and CD68 macrophages in intestinal mucosa of the rabbits in various groups were detected by immunohistochemistry;the levels of 17β-estradiol,lactic acid and IL-6 in serum of the rabbits in various groups were determined by ELISA method. Results: Two hours after shock treatment,the mesenteric blood flow state of the rabbits in anisodamine treatment group and 17β-estradiol prevention group was better than that in saline treatment group (P<0.05).In model group,the mucosal glands were atrophied,the villous columnar epithelial cells were powdered and corroded; in saline treatment group,the intestinal mucosal epithelium was partially exfoliated and broken,the villi structure was incomplete,and there were many exfoliated mucosal tissues in intestinal cavity; in anisodamine treatment group,the glands were slightly atrophied,the villi were slightly edema,and the lamina propria was slightly separated;in 17β-estradiol prevention group,the intestinal mucosal glands were normal,and the villi and the lamina propria were edema. The Chiu's score of the rabbits in model group was higher than those in other three groups (P<0.05);the Chiu's score of the rabbits in saline treatment group was higher than those in 17β-estradiol prevention group and anisodamine treatment group (P<0.05).The positive expression rates of IL-6 and CD68 macrophages in intestinal mucosa of the rabbits in anisodamine treatment group and 17β-estradiol prevention group were lower than those in saline treatment group and model group (P<0.05). The level of serum 17β-estradiol of the rabbits in 17β-estradiol prevention group was significantly higher than those in the other groups (P<0.05); the levels of serum lactic acid and IL-6 of the rabbits in 17β-estradiol prevention group were lower than those in the other groups (P<0.05);the levels of serum lactic acid and IL-6 of the rabbits in anisodamine treatment group and 17β-estradiol prevention group were lower than those in saline treatment group (P<0.05). Conclusion: 17β-estradiol can improve the intestinal mucosal microcirculation injury in the HS rabbits,and its mechanism may be realized by reducing the production of lactic acid,reducing the infiltration of macrophages, and inhibiting the release of inflammatory factor IL-6.

Key words: 17β-estradiol, hemorrhagic shock, intestinal mucosa, microcirculation, rabbit

CLC Number:

R605.971

YANG Lijuan, XIA Xueyi, WANG Zhenjiang, WU Dianxiu, GE He, ZHANG Ying, LIN Jiayu, ZHAO Peng, JING Qi, ZHANG Hanyuan, LIU Yanbo. Improvement effect of 17β-estradiol on intestinal mucosal microcirculation in rabbits with hemorrhagic shock and its mechanism[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1199-1205.

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Improvement effect of 17β-estradiol on intestinal mucosal microcirculation in rabbits with hemorrhagic shock and its mechanism

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