牙髓炎模型大鼠血清褪黑素水平及其对牙髓炎症反应的抑制作用

doi:10.13481/j.1671-587x.20190624

Abstract

Abstract: Objective: To investigate the effect of serum melatonin level in the rats with pulpitis on the nucleotide-binding oligomerization domain-like receptor protein 3/cysteine-containing aspartate proteolytic enzyme 1 (NLRP3/caspase-1) signaling pathway,and to explore the mechanism of the effect of melatonin on pulpitis. Methods: A total of 130 rats were divided into control group (n=40),pulpitis group (n=60) and pulpitis+melatonin group (n=30).Thirty rats with pulpitis were randomly divided into pulpitis 1 d group,pulpitis 3 d group and pulpitis 5 d group.The pulpitis rat model was established by pulpotomy exposure method, and the rats in pulpitis+melatonin group were intraperitoneally injected with melatonin after intramedullary surgery.HE staining was used to confirm the modeling of pulpitis;the serum levels of interleukin-1β(IL-1β) and melatonin and the levels of IL-1β in dental pulp tissue of the rats in various groups were detected by ELISA method, the expresion levels of NLRP3 and caspase-1 mRNA in dental pulp tissue of the rats in various groups were detected by RT-PCR method,and the expression levels of NLRP3 and caspase-1 proteins in dental pulp tissue of the rats in various groups were detected by Western blotting method. Results: The HE staining results showed that in control group,the dental pulp tissue of the rats was normal;in pulpitis group,the cavities in the dental pulp and a large number of inflammatory cells were found.The serum IL-1β levels of the rats in pulpitis 1 d group and pulpitis 3 d group were obviously higher than those in control group (P<0.05),and the serum melatonin levels were obviously lower than those in control group (P<0.05);the serum IL-1β level of the rats in pulpitis 3 d group was obviously lower than that in pulpitis 1 d group (P<0.05),and the serum melatonin level was obviously higher than that in pulpitis 1 d group (P<0.05).The levels of IL-1β in dental pulp tissue of the rats in pulpitis group and pulpitis+melatonin group were obviously higher than those in control group (P<0.05),the level of IL-1β in dental pulp tissue of the rats in pulpitis+melatonin group was obviously lower than that in pulpitis group (P<0.05). The expression levels of NLRP3 and caspase-1 mRNA and proteins in dental pulp tissue of the rats in pulpitis group and pulpitis+ melatonin group were obviously higher than those in control group (P<0.05),and the levels of NLRP3 and caspase-1 mRNA and proteins in dental pulp tissue of the rats in pulpitis+melatonin group were obviously lower than those in pulpitis group (P<0.05). Conclusion: The serum melatonin level in the rats with pulpitis is reduced,and melatonin can reduce the inflammatory response in dental pulp tissue of the rats with pulpitis by inhibiting NLRP3/caspase-1 signaling pathway.

Key words: pulpitis, melatonin, nucleotide-binding oligomerization domain-like receptor proein 3, cysteine-containing aspartate proteolytic enzyme 1, interleukin-1β

CLC Number:

R781.3

HAN Yaolun, WANG Lu, MA Xin. Levels of serum melatonin in rats with pulpitis and its inhibitory effect on dental inflammation[J].Journal of Jilin University(Medicine Edition), 2019, 45(06): 1334-1339.

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Levels of serum melatonin in rats with pulpitis and its inhibitory effect on dental inflammation

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