序贯培养体系和单一培养体系对人卵母细胞受精和早期胚胎发育的影响

doi:10.13481/j.1671-587x.20200101

Abstract

Abstract: Objective: To compare the effects of sequential and single-step culture media systems on the development of human early embryo in in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles, and to provide a reference for the selection and evaluation of the human embryo culture system of assisted reproductive technology (ART). Methods: A total of 155 patients received IVF/ICSI treatment were selected and the ova from the same patient were divided into two groups. The ova were cultured in Vitrolife sequential media system G-IVF/G1/G2 culture solution(sequential culture group) and Irvine single-step media system CSCC culture solution(single-step culture group)for IVF and embryo culture.The fertilization and early embryo development in the different culture systems in two groups were observed. Results: Compared with single-step culture group, the fertilization rate, 2PN fertilization rate and multi-nuclear fertilization rate of the patients in sequential culture group had no significant differences (P>0.05). In the IVF patients, there were no significant differences in the cleavage rates, quality embryo rates, high quality embryo rates and blastocyst formation rates between sequential culture group and single-step culture group (P>0.05). In the ICSI patients, there were also no significant differences in the cleavage rates, quality embryo rates and blastocyst formation rates between sequential culture group and single-step culture group (P>0.05). However, the high quality embryo rate of the patients in sequential culture group was significantly higher than that in single-step culture group (P=0.015). Both in the IVF and ICSI patients, the percents of densification embryos in single-step culture group were significantly higher than those in sequential culture group (P=0.001). Moreover, the embryos cultured in sequental culture group were smooth and homogeneous, but the embryos cultured in single-step culture group were rough and granular. Conclusion: There are no significant differences in fertilization and early embryo development between the two culture media systems.

Key words: culture system, sequential culture medium, single-step culture medium, in vitro fertilization, embryo development

CLC Number:

R321-33

LI Youzhu, YAN Xiaohong, WU Rongfeng, TAO Ping, CHENG Ling, YE Yuanyuan, ZHOU Weidong. Effects of sequential and single-step culture media systems on human oocyte fertilization and early embryo development[J].Journal of Jilin University(Medicine Edition), 2020, 46(01): 1-6.

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Effects of sequential and single-step culture media systems on human oocyte fertilization and early embryo development

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