电离辐射对小鼠骨髓造血干/祖细胞中CD47表达的影响

doi:10.13481/j.1671-587x.20200610

Abstract

Abstract: Objective

To investigate the damage of bone marrow hematopoietic stem/progenitor cells （HSCs/HPCs） and the changes of CD47 expression levels in the HSCs/HPCs of the mice after X-ray irradiation，and to clarify the role of CD47 in irradiation-induced injury of HSCs/HPCs.

Methods

The C57BL/6 mice were divided into nonirradation group and irradation group， and the C57BL/6 mice in irradation group were divided into 18 h， 5 d， 11 d， and 6 weeks after irradiation subgroups，and there were 5 mice in each group. The mice in irradation groups were given by an X-ray irradiator with a dose of 3.0 or 9.0 Gy at one time. The mice were sacrificed at different time points after irradiation.The percentages of HSCs to HPCs in bone marrow of the mice in various groups were detected by flow cytometry， and the expression levels of CD47 in HSCs/HPCs of the mice in various groups were detected at different time points after irradiation.

Results

The level of CD47 expression in HPCs of the mice in non-irradation group was more than 2-fold higher than the mature HSCs in bone marrow （P<0.05）. Compared with non-irradation group， there were no statistical differences in the CD47 expression levels in HPCs of the mice in irradation groups at 18 h， 5 d， and 11 d after irradation （P>0.05）； the percentages of HPCs in lineage-negative （lin^－） cells and whole bone marrow cells were decreased significantly（P<0.05）， which returned to normal level at 6 weeks after irradiation. Compared with non-irradiation group， the CD47 expression level in HSCs of the mice in 18 h after irradiation group was increased （P<0.01）， and there were no significant differences in the CD47 expression levels in HSCs of the mice in 5 d，11 d and 6 weeks after irradiation groups（P>0.05）； the percentage of HSCs in Lin^－ cells in 18 h after irradiation group was increased （P<0.01）， and the percentage of HSCs cells in whole bone marrow cells in 11 d after irradiation group was decreased （P<0.01）.The percentage of CD47 low expression subgroup was significantly lower than that of CD47 high expression subgroup in the HPCs in 18 h after irradiation group（P<0.01）.The percentage of CD47 low expression subgroup in the HPCs in 5 d，11 d and 6 weeks after irradiation groups were similar to those in CD47 high expression subgroup.

Conclusion

The total body ionizing radiation can cause the bone marrow damage in the mice and the decreasing in the percentage of HSCs to HPCs， and the mechanism may be related to the transient increase of CD47 expression in the HSCs after irradiation.

Key words: ionizing radiation, myelosuppression, hematopoietic stem cells, hematopoietic progenitor cells, CD47

CLC Number:

R144.1

Guangyao XIE,Feng WANG,Ting ZHANG,Yanhou LIU,Yongguang YANG. Effect of ionizing irradiation on expression of CD47 in bone marrow hematopoietic stem/progenitor cells in mice[J].Journal of Jilin University(Medicine Edition), 2020, 46(6): 1169-1176.

Figures/Tables 8

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Effect of ionizing irradiation on expression of CD47 in bone marrow hematopoietic stem/progenitor cells in mice

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