Journal of Jilin University Medicine Edition ›› 2013, Vol. 39 ›› Issue (3): 559-564.doi: 10.7694/jldxyxb20130328
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TAO Tao,CHEN Hong,GU Xue-shuang,ZHOU Jun-hao,PENG Dan-yi,ZHANG Li-jun
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Abstract: Abstract:Objective To investigate the role of vascular endothelial cadherin (VE-cadherin) on the angiogenesis of non-small cell lung cancer(NSCLC),and to provide experimental basis for anti-angiogenic therapy of NSCLC.Methods The A549 cells were divided into blank control group,interference group and negative control group.VE-cadherin-siRNA was transiently transfected into A549 cells,and Real-time quantitative PCR and Western blotting methods were used to detect the expression levels of VE-cadherin mRNA ;the changs of cell absorbance(A490) value was detected by four methy1 thiazolyl tetrazolium(MTS) assay;the cell cycle and apoptosis were detected by flow cytometry;the expression level of VE-cadherin in transiently transfected A549 cells supernatant was detected by ELISA method;the changes of the A490 value,ratios of cell cycle(G0/G1,G2/M and S),apoptotic rate and Matrigel tubule formation number of human umbilical vein endothelial cells(HUVECs) were analyzed after treated with supernatant.Results The expression level of VE-cadherin mRNA in interference group (0.299±0.039) was significantly lower than those in blank control group(1.137±0.082) and negative control group (1.001±0.076) (P<0.05);the expression level of VE-cadherin protein in interference group(0.297±0.045)〖JP2〗 was significantly lower than those in blank control group (0.833±0.119) and negative control group (0.794±〖JP〗0.095)(P<0.05).The A490 values,cell cycle ratios and apoptotic rates of the A549 cells in blank control group,interference group and negative control group had no significant differences(P>0.05);the expression level of VE-cadherin in the supernatant in interference group was (2.89±0.07)μg/L,which was significantly lower than those in blank control group [(11.43±0.09) μg/L] and negative control group [(11.15±0.04) μg/L] (P<0.05).Between interference group and negative group,the percentages of HUVECs at G0/G1,G2/M and S phases had no significant difference after treated with supernatant(P>0.05);but the proliferation of HUVECs in interference group was inhibited by supernatant significantly,the inhibitory rates of cell proliferation 24,48 and 72 h after treatment were 29.2%,33.8%,and 30.1%,respectively.48 h after treatment,the apoptotic rate of the HUVECs in interference group was 27.12%±5.22%,which was significantly higher than that in negative control group (13.43%±3.50%) (P<0.05).The number of tubule formation was 1.53±0.31,which was significantly lower than that in negative control group(14.53±1.51)(P<0.05). Conclusion VE-cadherin may participate in the occurrence and development of NSCLC by promoting angiogenesis,which is expected to become a new target for anti-angiogenic therapy of NSCLC.
Key words: vascular endothelial cadherin, angiogenesis, cancer,non-small cell lung, human umbilical vein endothelial cells
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TAO Tao,CHEN Hong,GU Xue-shuang,ZHOU Jun-hao,PENG Dan-yi,ZHANG Li-jun. [J].Journal of Jilin University Medicine Edition, 2013, 39(3): 559-564.
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URL: http://xuebao.jlu.edu.cn/yxb/EN/10.7694/jldxyxb20130328
http://xuebao.jlu.edu.cn/yxb/EN/Y2013/V39/I3/559
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