mIL-12重组逆转录病毒载体的构建及包装细胞系的建立

doi:吉林省科技厅资助课题（20030542-1）；长春

• 基础研究 • Previous Articles Next Articles

Construction of mIL-12 recombinant retrovirus vector and establishment of viral package cell line

LI Jing-hua¹,LI Jing-hua¹, CHEN Dong^2*，LIU Ying²LIU Ying²

1.Department of Pathogenobiology, School of Basic Medical Sciences,Jilin University,Changchun 130021,China；2. Department of Histology and Embryology, School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2005-04-07 Revised:1900-01-01 Online:2006-03-28 Published:2006-03-28
Contact: CHEN Dong^2*

Abstract

Abstract: Objective To construct mIL-12 recombinant retrovirus vector and evaluate the effect of mIL-12 expression on glioma in rats. Methods mIL-12 DNA was cloned into vector pLEGFP using standard procedures to develop recombinant plasmid pLEGFP-mIL 12 ,then it was transferred into PA317 cells. Results Products of enzyme digestion and PCR of recombinant plasmid pLEGFP-mIL12 were analysed using electrophoresis and a 2 290 bp fragment of DNA as same as mIL-12 gene in size was found, transfected NIH3T3 expressed GFP-mIL12 fusion protein. ls can produce retrovirus which can infect NIH3T3 cells,and the expression of mIL-12 gene can be detected in NIH3T3 cells.

Key words: retroviridae, package cell

CLC Number:

LI Jing-hua,LI Jing-hua, CHEN Dong，LIU YingLIU Ying. Construction of mIL-12 recombinant retrovirus vector and establishment of viral package cell line[J].J4, 2006, 32(2): 254-256.

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Construction of mIL-12 recombinant retrovirus vector and establishment of viral package cell line

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