Abstract:

Abstract:Objective To  study the effects of lactate peritoneal dialysis solution(L-PDS) with different concentrations on apoptosis of human peritoneal mesothelial cells(HPMC),the expressions of bcl-2,bax and activity of caspase-3.Methods HPMC were separated using enzyme digestion and cultivated stably in vitro.After HPMC were co-cultivated with different concentrations(1.50%,2.50%,4.25%) L-PDS,flow cytometry  was used to test the apoptosis  of HPMC,RT-PCR was used to observe the expressions of bcl-2 and bax,fluorometric method was used to detect the activity of caspase-3.
Results Compared with control group, L-PDS could induce the apoptosis of HPMC,especially in high concentration.With the increasing of  L-PDS concentration,the expression of bcl-2 mRNA decreased,the expression of bax mRNA increased,the activity of caspase-3 raised.There were significant differences of the indexes mentioned above between 4.25%,  2.50% L-PDS groups and  control group（P<0.05），there also was significant difference between 4.25% L-PDS group and  2.50% L-PDS group(P<0.05)，but there was  no significant difference  between 1.5% L-PDS group and  control group(P>0.05).
Conclusion L-PDS could induce HPMC apopto9sis,which may be executed by alternating of the expressions of bcl-2,bax and activating of caspase-3.

Key words: lactate peritoneal dialysis solution;human peritoneal mesothelial cells;apoptosis;genes,bcl-2;bax;caspase-3