Abstract:

Abstract:Objective To study the effects of eukaryotic expressive mature peptide LL-37 of human cationic antimicrobial peptide (hCAP-18) on the expressions of membrane molecules on dendritic cells (DCs). Methods By gene cloning,the eukaryotic expressive plasmid pcDNA4/Myc-His-LL-37 for the mature peptide LL-37 of hCAP-18 was constructed.Then they were transfected into HEK293 cell lines.After the cell lines were cultivated for 48 h，the supernatant was collected.Then the DCs from peripheral blood mononuclear cells (PBMCs) induced by rh-GM-CSF and rh-IL-4 were cultivated with the supernatant for 48h.The expressions of membrane molecules CD40 and HLA-DR on DCs were detected by flow cytometry (FCM).
Results  The eukaryotic expressive plasmid pcDNA4/Myc-His-LL-37 was constructed successfully and it expressed in eukaryotic cells HEK293.FCM results indicated that the expressions of CD40 and HLA-DR on the membrane of DCs which were stimulated by the supernatant produced by pcDNA4/Myc-His-LL-37 were higher than those in  control group(P<0.05). Conclusion The mature peptide LL-37 of hCAP-18 is constructed successfully and it can increase the expressions of the  membrane molecules on DCs such as CD40 and HLA-DR.

Key words: hCAP-18/LL-37;mature peptide;dendritic cells;membrane molecules