Abstract:

Abstract:Objective To investigate the effects of nerve growth factor (NGF) on metabolism and function  of neuroglial cells in the normal mice and the nonobese diabetic (NOD) mice.Methods  The mice were  divided into normol group and NOD group (n=10).The neuroglial cells  abstracted from the normal mice and the NOD mice were randomly divided into control  (with RPMI-1640) and 4 experimental groups (n=10);5,10 and 20 μg·L^-1NGF and 20 μg·L^-1LPS +10 μg·L^-1NGF were used in experimental groups.The activity of superoxide dismutase(SOD) and malonic dialdehyde(MDA) content in the culture solution were detected with xanthine oxidase and TBA methods.
Results Compared with control group of normal mice,the  activity of SOD and MDA content in neuroglial cells  of the NOD mice were significantly　decreased (P<0.01)；compared with that of respective control group,the activities of SOD were increased (P<0.05)and the MDA contents were decreased  significantly (P<0.05) in    the NOD mice of NGF groups.
Conclusion  NGF has some effects on the improvement of cell metabolism and function in the NOD mice.

Key words: nerve growth factor；neuroglial cells； superoxide dismutase；malonic dialdehyde； nonobese diabetic mice