Abstract:

Abstract:Objective To observe the expression of matrix metalloproteinase-2 (MMP-2) in U2OS cells transfected with human telomerase reverse transcriptase (hTERT),and explore the dependability between and  elomerase,and study the effect of telomerase activation in tumor infiltration and metastasis and their possible mechanisms of action. Methods  Recombinant plasmid PCI-Neo-hTERT  was  transfected into human osteosarcoma cell line U2OS.Two transfected positive clonings were chosen for experiment,while normal U2OS cells were used as control group.The effect of hTERT transfected into U2OS cells was detected by RT-PCR and TRAP-ELISA assays,zymography and RT-PCR were used to observe the expression and activity of MMP-2.Results Compared with control group,two positive clonings ectopic generated hTERT mRNA and expressed telomerase activity successfully(ΔA>0.2 U),while the MMP-2 mRNA without obvious difference(P>0.05),and the expression of proMMP-2  was decreased obviously(P<0.01).Conclusion Human osteosarcoma cell line U2OS transfected with hTERT could generate telomerase activity,moreover，the appearance of telomerase activity could regulate the secretion  of MMP to affect the degradation and construction of ECM which can affect tumor infiltration and metastasis.

Key words: human telomerase reverse transcriptase gene；U2OS cells；telomerase activity;matrix metalloproteinases