Abstract:

Abstract:Objective To study the inhibitory effect of phenylacetate(PA) on hepatic carcinoma cells for developing of gene therapy. Methods SSMC-7721 cells  were treated with or without PA (0.25,0.50,1.00,2.00,4.00 mmol·L^-1) for 24,48 and 72 h,respectively. The cellular proliferation inhibitory rate was evaluated by MTT assay. Flow cytometry was used to estimate the changes of cell cycle. The morphous of tumor cells suppressed by PA was observed with electronic microscope. Finally,the activity of Caspase-3 protein  to estimate the way of apoptosis of SSMC-7721 cells under the control of PA was determined.Results The growth of SSMC-7721 cells were inhibited by 0.25-4. 00 mmol·L^-1 PA for 24-72 h.With the increase of concentration of PA, the proliferation inhibitory rate were 7.2%-73.1 % at 24 h, 9.1%-87.5% at 48 h, 15.8%-91.9% at 72 h.With the prolongation of  treating time and the increasing  of  PA concentration, the inhibitory rate was increased. 2.00 mmol·L-1 was the best concentration for the experiment. The cell cycle was arrested at G0 / G1 and G2/M phase. Some characteristic changes of apoptosis were observed  by electronic microscope,the activity of Caspase-3 protein in SSMC-7721 mediated by PA was increased.Conclusion PA could effectively inhibit SSMC-7721 cell proliferation and induce the apoptosis. Caspase-3 protein at least partly participate in the mechanism.

Key words: phenylacetate, hepatic carcinoma cells, suppression