Abstract:

Abstract:Objective To explore the effect of modified amantadine(NAM) on immune function of the mice infected with influenza A(H5N1) virus and to provide experimental basis for development of NAM.Methods Mouse models infected with influenza A (H5N1) virus were established.And the mice were divided into 7 groups:normal control group（NC）,positive control group (PC),amantadine group (AMA) and 4 NAM groups (which contained 25,50,100 and 200 mg?kg-1 NAM, respectively).The spleen and thymus index,T lymphocyte transformation,NK cytotoxicity activity and interferon (IFN) activity of mice were detected.Results The  mean spleen index in 100 mg?kg-1 NAM group was higher than that in PC group (P<0.05),and the mean thymus index in NAM group was higher than that in PC group (P<0.05).The spleen indexes  in 25,100 and 200 mg?kg-1 NAM groups were increased compared with PC group(P<0.05),and were and increased in 100 and 200 mg?kg-1 NAM groups compared with AMA group (P<0.05).The NK cytotoxicity activities in 100 and 200 mg?kg-1 NAM groups were   higher than that in PC group(P<0.05),while they were also higher in 25,100 and 200 mg?kg-1 NAM groups than that in AMA group (P<0.05).The IFN activities in 50,100 and 200 mg?kg-1NAM groups were increased compared with PC group(P<0.05),and the IFN activities in 50,100 and 200 mg?kg-1 NAM groups were also increased compared with AMA group (P<0.05).
Conclusion NAM can stimulate the proliferation of spleen and thymus cells of mice infected with influenza A(H5N1) virus,and enhance T lymphocyte transformation,NK cytotoxicity  and IFN activity.

Key words: avian influenza virus;amantadine;immune function