Abstract:

Objective To detect the expressions of Bcl-2 and Bax gene and protein  in myocardium of rats with isoproterenol (Isp)-induced myocardial necrosis and significances.Methods 40 Wistar rats were divided into control group,12 h,1 week and 3 weeks  after Isp injection groups.Except control group,all the rats in the other groups were treated with isoproterenol hydrochloride.12 h,1 week and 3 weeks after Isp injection,immunohistochemistry and RT-PCR were applied to detect the gene and protein expressions of Bcl-2 and Bax.Results The results of immunohistochemistry showed that  12 h and 1 week after Isp injection,the expressions of Bax were increased significantly compared with control group (P<0.05);Inversely,the expressions of Bcl-2 were decreased significantly 12 h,1 week and 3 weeks after Isp injection compared with normal control group (P<0.05).The ratios of Bcl-2/Bax were significantly decreased  12 h,1 week and 3 weeks after Isp injectioncompared with control group (P<0.05). The expressions of Bax mRNA were increased significantly
 12 h,1 week and 3 weeks after Isp injection compared with control group (P<0.05);The expressions of Bcl-2 were decreased significantly 12 h and 1 week after Isp injection compared with control group (P<0.05).Conclusion Apoptosis of myocardium is the basic pathological change of myocardial fibrosis,and the expressions of gene and protein of Bcl-2 family  play an important role in this process.

Key words: myocardial necrosis;apoptosis;gene,bcl-2;Bax