Abstract:

Objective
To investigate the expressions of FSH receptor mRNA and protein in ovary tissue in rats with  letrozole-induced polycystic ovary syndrome(PCOS),and to provide experimental data for the model application. Methods Forty rats were randomly divided into two groups(n=20),in PCOS model group letrozole  was administered once daily during 21 d,and  in control group  without any treatment.The  gonadal hormone concentrations in serum were determined by radioimmunoassay,the histologic changes in ovaries were observed by HE staining,the expression of FSH receptor gene in ovary tissue was detected by realtime -PCR,Western blotting and immunohistochemistry.
Results  Compared with control group,estradiol(E2) and progesterone in model group showed a considerable reduction（P<0.05）,the serum testosterone (T) and follicl
e-stimulating hormone (FSH) and luteinizing hormone(LH) levels in model group were markedly increased than those in control group（P<0.05）.The ovarian weights of the two groups had no significant difference(P>0.05).Compared with control group,the ovaries from model group showed high incidence of subcapsular ovarian cyst and capsular thickening and decreased number of corpora lutea.The expressions of FSH receptor mRNA and protein were significantly higher in model group than those in control group（P<0.05）.Conclusion The expression of FSH receptor gene in letrozole-induced polycystic ovaries is similar with that of PCOS women,the rat model is proved to be an ideal PCOS animal model to study the pathophysiology of PCOS.

Key words: polycystic ovarian syndrome；disease model,animal；letrozole；receptors,FSH