J4 ›› 2009, Vol. 35 ›› Issue (6): 1074-1078.
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GONG Cui-Cui1, ZHENG Nai-Gang2,3, WU Jing-Lan3, LAI Ze-Ren1, HE Pei-Xia1
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Abstract:
Objective To explore the antioxidant effect of honeysuckle on hepatic RBL cells of rats in vivo and in vitro and its underlying mechanism,and provide theoretical basis for the application of honeysuckle in clinic.Methods The blood levels of T-AOC,SOD,GSH-Px,GSH or MDA in an antioxidant enzyme system were detected 1-2 h after administration in the established rat models,which were administrated with larger and smaller doses of honeysuckle respectively.The cultured human hepatic RBL cells were exposed to H2O2 as an oxidant and treated with 0.25g?L-1 neysuckle before or after exposure to H2O2.The cell survival and apoptosis were detected by MTT and TUNEL assays.The expression levels of NF-kB,Bcl-2,Bax,HSP-70 and Caspase-3 were determined by immunohistochmistry and Western blotting and the cell anti-oxidant enzyme system was detected by corresponding kits.Results In the antioxidant and anti-apoptotic effects of honeysuckle,the pre-protective effect was markedly higher than the post-protective.The expressions of NF-κB and HSP-70,the expression ratio of Bcl-2/Bax were down-regulated,and the apoptotic rate in pr e-protective group was lower than that in post-protective group. Besides,the detected cell level of anti-oxidant enzyme system was up-regulated significantly.In the plasma of rat model the levels of T-AOC,GSH-Px,GSH and SOD were promoted(P<0.05),while that of MDA was decreased markedly(P<0.05).Conclusion Honeysuckle displays antioxidant capacity through up-regulating the antioxidant enzyme system and down-regulating NF-κB signal transduction pathway.
Key words: honeysuckle;hydrogen peroxide;antioxidant enzyme system; anti-apoptoic signal transduction;human hepatic RBL cell line
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GONG Cui-Cui, ZHENG Nai-Gang, WU Jing-Lan, LAI Ze-Ren, HE Pei-Xia. Antioxidant effect of honeysuckle on hepatic RBL cells of |rats in vivo |and in vitro and mechanism[J].J4, 2009, 35(6): 1074-1078.
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