Abstract:

Abstract:Objective　To study the change of c-myc gene expression in thymic lymphomas of mice induced by ionizing radiation and provide theoretical basis for     mechanism of radiation carcinogenesis.Methods　The thymic lymphomas models of BALB/c mice were made by   X-ray(dose rate:0.287 Gy?min-1;total dose:7 Gy) exposuring,6 months later they were killed,then total RNA  and  total protein were extracted  from thymic lymphomas a
nd normal thymus tissue and cDNA was synthesized, the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively.Results　The expression of c-myc mRNA  in thymic lymphomas（9.16±4.66） was higher than that in normal thymus tissue（1.16±0.54）（P<0.01）. The expression of c-myc protein in thymic lymphomas was higher than that in normal thymus tissue. Conclusion　There is relevance between c-myc gene and thymic lymphomas induced by ionizing radiation，c-myc gene might be susceptibility gene of radiation carcinogenesis.

Key words: thymic neoplasms;lymphoma;genes, myc;quantitative real-time PCR;ionizing radiation