Abstract:

Abstract:Objective To determine the protective effect of cardiopulmonary bypass(CPB)  with autologous lung as oxygenator on CPB-relative inflammatory response.Methods Twelve adult mongrel dogs were randomly divided into control group and experimental group.CPB using a membrane oxygenator (control group) or using the autologous lung (experimental group) for gas exchange was performed for 120 min in an alternating series of 12 mongrel dogs with 90 min heart arrest by crystalloid cardioplegia and 30 min reperfusion.The blood samples were collected at the same time point of pre-operation(T1)，60 min of CPB(T2)，and 1 h (T3),2 h (T4) after CPB．The plasma levels of IL-6，IL-10,TNF-α were detected with ELISA．  Results The plasma levels of IL-6，IL-10 and TNF-α of dogs in each group were significantly increased at T2,T3 and T4(P<0.01) compared with before CPB.The plasma levels of IL-6 and TNF-α in experimental group were significantly lower than those in control group at T2,T3 and T4(P<0.01).The plasma levels of IL-10 in experimental group were significantly higher than those in control group at T2,T3 and T4(P<0.05).Conclusion Extracorporeal circulation with autologous lung as oxygenator could reduce the increased amplitude of the plasma levels of TNF-α and IL-6，whereas enhance the increased amplitude of the plasma IL-10 level.The results indicate that extracorporeal circulation with autologous lung as oxygenator can reduce  the CPB-relative inflammatory respones.

Key words: autologous oxygenator;inflammatory response；cytokines;cardiopulmonary bypass