Abstract:

Abstract:Objective To  compare the changes of   myocardial morphology,myocardial enzymology and hemorheology  between the acute myocardial infarction models  with blood stasis and the simple acute myocardial infarction models in rats  and elucidate the difference between the acute myocardial infarction models in rats with blood stasis and the simple acute myocardial infarction models in evaluation of pharmacodynamics.Methods Wistar rats were randomly divided into  control,acute blood stasis model,acute myocardial infarction sham operation,acute myocardial infarction model and  acute myocardial infarction model with blood stasis groups.The acute myocardial infarction models under the status of the acute blood stasis in rats were built to determine the parameters of myocardial infarct size (MIS) and the activities of serum aspartate aminotransferase (AST),creatine phosphokinase (CK) and lactate dehydrogenase (LDH),at the same time,platelet agglutinate rate (PAR),platelet aggregation(PAG),erythrocyte sedimentation rate(ESR),hematocrit(HCT) and the length,the dry weight,the wet weight of the thromb in vitro were tested,the parameters of thrombelastogram were also be detected.Results ①When the acute myocardial infarction models with blood stasis in rats and the simple acute myocardial infarction models were comparatively studied,the results showed that there were no obvious differences between them in MIS,the activities of CK,AST and LDH,and PAR and PAG（P>0.05）;② The increasing extent of the ESR of the acute myocardial infarction models with blood stasis in rats was much higher than that of the simple acute myocardial infarction models（P<0.05）,but there was no obvious difference between them in HCT（P>0.05）;③ The dry weight and the wet weight of the thromb in vitro of the acute myocardial infarction models with blood stasis in rats were  much heavier those  of the simple acute myocardial infarction models and the acute blood stasis syndrome models in rats（P<0.05 or P<0.01）,but there was no obvious difference between them in the length of thromb in vitro（P>0.05）;④ The increasing extent of the values of r,k and the reducing extent of the value of ma in the acute myocardial infarction models with blood stasis in rats were significantly higher than those in the simple acute myocardial infarction models（P<0.05）,but there was no obvious difference between the acute myocardial infarction models with blood stasis and the acute blood stasis syndrome models in rats（P>0.05).Conclusion The obvious differences are showed in the parameters of blood sedimentation and the weight of the thromb in vitro and the thrombelastogram,but there are no obvious differences in the indexes of MIS,the myocardium enzyme in  serum,HCT and the index of  function of the blood plate when the acute myocardial infarction models with blood stasis and the simple acute myocardial infarction models are comparatively studied.The results  show that it is  defective to evaluate pharmacodynamics of traditional Chinese drug only with  simple acute myocardial infarction models.

Key words: blood stasis;myocardial infarction;disease models,aninal;rats,Wistar