Abstract:

Abstract:Objective To illustrate the genotypes and distribution of the extended-spectrmu β-lactamases (ESBLs) of gram-negative bacterial isolates from several hospitals in Changchun,Jilin,China and to provide the reliable basis for antibiotic application. Methods Drug-resistant genes associated with ESBLs in gram-negative bacterial isolates were amplified by PCR. PCR products were sequenced and compared with the genotypes reported in  GenBank.Results  68 strains of 165 isolates exhibited drug resistance such as  TEM-type (12.12%),CTX-M-1 type (4.24%),SHV-type (3.64%) and CTX-M-9 type (3.03%).The multi-drug resistant genes including SHV + TEM-type,TEM + CTX-M-1 type,TEM + CTX-M-9 type,TEM + CTX-M-1 + CTX-M-9 type and SHV + TEM + CTX-M-1 + CTX-M-9 type were also detected. The positive PCR amplification products were  randomly selected and  sequenced,the SHV-11 subtype,TEM-1 subtype,CTX-M-3 subtype and the CTX-M-15 subtype,CTX-M-14 and CTX-M-18 subtype were obtained. Conclusion The genotypes of ESBLs in gram-negative bacterial  isolates mainly include TEM-1 subtype,CTX-M-14 subtype,CTX-M-18 subtype,SHV-11 in Changchun area.

Key words: extended-spectrum β-lactamases;gram-negative bacilli;polymerase chain reaction