Abstract:

Abstract:Objective To study the mechanism of the changes of proliferation rate induced by the proteasome inhibitor Lactacystin(LAC)   through  observing the effect of    LAC on the proliferation rate of glioma C6 cells, and provide a theoretical basis for clinical treatment of glioma. Methods The cultured rat glioma C6 cells at the logarithmic growth phase were divided into control group,LAC 2.5 μmol/L group,LAC 5.0 μmol/L group and LAC 10.0 μmol/L Group.MTT assay was used to detect the proliferation rate of C6 cells,FCM was used to detect the death and mitochondrial membrane potential of C6 cells and RT-PCR was performed to examine the Bax and bcl-2 mRNA expressions,Western blotting was used to determine the Bax,bcl-2 and P65 protein expressions.Results Compared with  control group,the proliferation rates of C6 cells,mitochondrial membrane potential and  the level of P65 protein expression in LAC 5.0  and LAC 10.0 μmol/L groups were  reduced (P<0.05),while the apoptotic rate,Bax/bcl-2 mRNA and protein ratio were increased (P<0.05).Conclusion  LAC can inhibit the proliferation rate of C6 cells by inclucing the apoptosis of C6 cells,which may be related to the mitochondrial pathway,in addition,NF-κB signaling pathway may also affect the proliferation rate of the C6 cells in this process.

Key words: Lactacystin;apoptosis;mitochondria;cell proliferation