Abstract:

Abstract:Objective To investigate the influence of purine nucleotide on adenosine deaminase (ADA) activity and gene expression in testis and epididymis tissues of heroin dependence and withdrawal rats.Methods The rat models of heroin dependence and withdrawal were set up.80 male Wistar rats were randomly divided into 8 groups (10 rats of each group):control group(C),nucleotide group(N),heroin administration group(H),heroin and nucleotide group(HN),3 d withdrawal group(W3),9 d withdrawal group(W9),nucleotide 3 d group(N3),nucleotide 9 d group(N9).The adenosine deaminase activity and gene expression in testis and epididymis tissues of rats in various groups were detected.Results Compared with control group,the ADA activities in heroin administration group and 3 d withdrawal group in testis and epididymis tissues were increased significantly (P<0.05);there was no obvious  difference of ADA activity between other groups and control group（P>0.05）.The ADA mRNA level in testis tissue in heroin group was significantly higher than that in control group (P<0.05);the ADA mRNA levels in epididymis tissue in heroin group,3 d withdrawal group and 9 d withdrawal group were significantly higher than that in control group (P<0.05);and there was no obvious difference of ADA mRNA level between other groups and control group（P>0.05）.Conclusion Heroin can enhance constantly the adenosine deaminase activity and gene expression in testis and epididymis tissues of rats.Purine nucleotide compensation can oppose the effect of heroin.

Key words: heroin；purine nucleotides；adenosine deaminase；testis；epididymis