Abstract:

Abstract:Objective To investigate the effects of different concentrations of lithium(LiCl) on neonatal rat cardiomyocytes exposed to high glucose,and study the  protective effects of canonical Wnt signaling pathway.Methods The ventricular myocardium of  neonatal rats about 1-3 d old was obtained.Then the primary culture cardiomyocytes were randomly divided into 4 groups:control group,high glucose damaged group,LiCl  5 and 10 mmol/L group.The morphological of cardiomyocytes were observed by inverted microscope,the apoptotic cardiomyocytes were tested by terminal deoxynucleotidyl transferase mediated dUTP_biotin nick end labeling(TUNEL),immunocytochemistry staining was used to detect the protein experssions p-GSK-3β and β-catenin.Results  Compared with high glucose damaged group, the pulsation frequency of cardiomyocytes tended towards nomal,the apoptotic cardiomyocytes were decreased（P<0.05）,the expressions of p-GSK-3β and β-catenin were increased（P<0.05）with the increasing of the concentration of LiCl in LiCl 5 and 10 mmol/L groups.Conclusion  LiCl can significantly inhibit the injury of cardiomyocytes induced by high glucose,its mechanism may be related to the activation of canonical Wnt signaling pathway．

Key words: lithium chloride;glycogen synthase kinase-3 beta;β-catenin;Wnt signaling pathway;myocardial cells