Abstract:

Abstract:Objective To investigate the synthesis and secretion of collagen type Ⅰ(Col Ⅰ) in human adipose-derived stem cells(hADSCs) during osteogenic differentiation and induction,and approach the efficacy of hADSCs as seed cells for bone regeneration engineering.Methods hADSCs were separated,cultivated and amplified in vitro and were identified by cell phenotype detection. Multi-lineage differentiation potential was assessed by histological staining such as oil red O for lipid accumulation and alizarin red S for mineralization nodules during adipogenic and osteogenic induction. Osteogenic differentiation and induction group was divided into five subgroups:0,7,14,21 and 28 d groups. Alizarin red S staining,Van Gieson collagen fiber staining and Col Ⅰ immunofluorescence were used to detect the expression of Col Ⅰ. FV Viewer 1.7 software was used to analyze the data. Results The positive stainning of oil red O was found 14 d after adipogenic induction. For the osteogenic induction groups,the calcified nodules and the collagen fiber red staining were found in 21 d group. The expression of Col Ⅰ was found in 21 d group for all cells and  strongly positive expression in 28 d group. Compared with 0 d group,the expression of Col Ⅰ in osteogenic induction group was significantly increased from 7 d to 28 d（P<0.05). Conclusion During osteogenic induction of hADSCs,all cells have osteogenic capability and the hADSCs have outstanding capability of osteoinductive and osteogenesis. hADSCs may be useful in  clinical cell-based therapy for bone regeneration engineering.

Key words: adipose-derived stem cells;osteogenic  , induction;multi-lineage differentiation potential;collagen type Ⅰ