Abstract:

Abstract:Objective To investigate the effect of  oligodeoxynucleotide (ODN) on  differentiation of  rat bone marrow mesenchymal stem cells(BMSCs) to osteoblasts.Methods BMSCs at the  third generation  were incubated for 24 h before 12 different ODN were added.The levels of alkaline phosphatase(ALP),reflecting the degree of osteogenic differentiation were detected in four replicate wells of BMSCs stimulated with each ODN with different concentrations( 4.0,2.0,1.0 and 0.5 mg/L) at different time points(24,72 and 120 h).Results There were no significant differernces of the mean level of ALP between 4.0 and 0.5 mg/L ODN groups and PBS control group;but in 2.0 and 1.0 mg/L ODN groups,there were two ODN which could increase the ALP levels at different time points(24,72 and 120 h)(P<0.01). Conclusion Two ODN of 12 different ODN can promote the diffentiation of BMSCs to osteoblasts.It suggests that ODN with special sequence characteristic can significantly promote the differentiation of rat BMSCs to osteoblasts.

Key words: bone marrow mesenchymal stem cells；differentiation ；oligodeoxynucleotide；osteoblasts