J4 ›› 2010, Vol. 36 ›› Issue (4): 656-659.
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HE Ming-rui1, DONG Yu2, FU Tong3, SUN Min-Dan2
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Abstract:Objective By observing the expressions of vascular endothelial growth factor (VEGF),metallothionein(MT),and matrix metalloprteinase-9 (MMP-9) in the kidneys of normal and diabetic rats,the influences of exogenous Zn in kindey and the expessions of VEGF,MT and MMP-9 in kidney tissues of diabetic rats were explored. Methods Forty-eight Wistar rats were randomly divided into four groups(n=12) : normal control (NC) group,negative control (STZ)group,external Zn intervention diabetic (STZ+Zn) group and external Zn intervention normal (Zn) group. The rat diabetic models were made through intraperitoneal injection of streptozotocin(STZ) in STZ and STZ+Zn groups.The rats in STZ+Zn and Zn groups were orally injected with 5 mg?kg-1of Zn sulphate twice a week. After fed for 16 weeks,the kidneys of rats were obtained and organ coefficient of kidney was calculated.The histopathological changes of kindeys and the expressions of MMP-9,VEGF and MT in kidney tissues were detected with immunohistochemistry method. Results Compared with NC group,the organ coefficients in STZ group and STZ+Zn group were increased (P< 0.05). Compared with STZ group,the organ coefficient in STZ+Zn group was increased(P<0.05). There was no difference of organ coefficient between STZ group and Zn group (P>0.05).The MMP-9,VEGF, and MT expressed in kidneys of rats.MMP-9 and VEGF mainly expressed in the distal renal tubule;and MT mainly expressed in the proximal renal tubulei. there was no difference of the expressions of MMP-9,VEGF, and MT in kidney tissues between various groups(P>0.05).Conclusion External Zn intervention can increase the organ cofficient of kidney in diabetic rats,but dosen’t affect the organ coefficient of kindey in normal rats. The expressions of MMP-9,VEGF, and MT in kidney tissues of rats have no changes after external Zn intervention.
Key words: vascular endothelial growth factor;metallothionein, matrix metalloproteinase-9;Zn;diabetes mellitus,experime
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HE Ming-rui, DONG Yu, FU Tong, SUN Min-Dan. Effects of Zn on |kidney of dibetic rats and expessions of VEGF,MT and MMP-9 in kidney tissues[J].J4, 2010, 36(4): 656-659.
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