Abstract:

Objective
To investigate the expressions of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor (VEGF) in ovarian cancer tissues and detect microvascular density (MVD) and elucidate the correlations between MIF,VEGF and the occurrence of ovarian cancer and explore their roles in tumor angiogenesis.Methods The expressions of MIF,VEGF and factor Ⅷ related antigen in normal ovarian tissues and ovarian cancer tissues were detected by immunohistochemical staining.The results were analyzed by Image Pro Plus（IPP）6.0 software.Results There were no visible expressions of MIF and VEGF in normal ovarian tissues,but positive expressions in ovarian cancer.The expressions of MIF and VEGF were increased significantly with the decreasing grade of the ovarian cancer.There were significant differences of the IOD values between well-differentiated,moderately differentiated and poorly differentiated ovarian cancer（P<0.05 or P<0.01）.The MVD in poorly differentiated ovarian cancer tissues (50.83±11.59) was significantly higher than those in normal ovarian tissues(18.16±2.26),well differentiated(19.88±3.92),moderately differentiated(31.1±3.08)  ovarian cancer tissues(all P<0.01).There was positive correlation between the expressions of MIF and VEGF in ovarian cancer tissues (r=0.579　7，P<0.01).Moreover,the expression of MIF was positively correlated with MVD in ovarian cancer tissues (r=0.962　3，P<0.01).Conclusion MIF plays an important role in the oncogenesis and development of ovarian cancer,and it may promote the angiogenesis of ovarian cancer tissues by up-regulating the expression of VEGF.

Key words: macrophage migration inhibitory factor;vascular endothelial growth factor;microvascular density;angiogenesis;ovarian cancer