Abstract:

Objective
To establish the high performance liquid chromatography  (HPLC) method for determination of  2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glucoside in Taohong Qingxue Capsule,and provide  basis for   the quality control of industrial production.Methods The HPLC method   was developed to determine 2,3,5,4 ′-tetrahydroxy-stilbene-2-O-β-D-glucoside in Taohong Qingxue Capsule. The developed conditions of HPLC were as follows: the shim-pack VP-ODS C18(150.0 mm×4.6 mm,5 μm) was used,the mobile phase was Acetonitrile - water（17∶83）,the detection wavelength was at 322nm,and the column temperature was 30℃.Results The linear range of 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glucoside was 0.284-2.272  μg.The regression equation was: Y=－25 678+1 204 880X，r=0.999 9.The average recovery was 99.4%,and the (RSD) was 2.29% (n=6). The RSD in accuracy,stability and repeatability tests  were 0.46%,2.89%, and 2.39%,respectively.The content of 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glucoside in Taohong Qingxue Capsule was limited not less than 0.8 mg per one capsule under the developed condition. Conclusion This method is accurate，simple and reliable,which provides the basis for  quality control of Taohong Qingxue Capsule.

Key words: Taohong Qingxue Capsule；2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glucoside；chromatography,high pressure liquid