Abstract:

To investigate the inhibitory effect of 20(S)-proto-panaxdiol（PPD）on the growth of siha cells in vitro and detect the transcription and expressions of autophagy related gene Beclin 1 and MAP1-LC3,and clarify the mechanism of its suppressing effect on siha cell proliferation. Methods The siha cells cultivated in vitro were treated with alcohol,40 μmol·L^-1 platinol (Pt),10 μg·L^-1 PPD,20 μg·L^-1 PPD,40 μg·L^-1 PPD for 24,48,72 and 96 h, respectively. The inhibitory rate of cell proliferation in each group was detected by MTT,the transcription and expressions of autophagy related gene Beclin 1 and MAP1-LC3 were analyzed by RT-PCR,Western blotting and immunocytochemical staining. Results After treated with 10,20 and 40 μg·L^-1 PPD,the inhibitory rates of siha cells were significantly increased compared with control group(P<0.05),and the inhibitory rate was  increased gradually with the time prolongation of time and the increase of dose. The transcription and expressions of autophagy related gene Beclin 1 and MAP1-LC3 were significantly up-regulated (P<0.01). Conclusion PPD may suppress the proliferation of siha cells,and its mechanism may be related to  up-regulating the expressions of autophagy related gene Beclin 1 and MAP1-LC3.

Key words: 20(S)-proto-panaxdiol;siha cell;autophagy;Beclin 1;MAP1-LC3