Abstract:

To investigate the expression of tissue transglutaminases (tTG) in rat ischemic myocardium and the role of sodium ferulate (SF) in rat myocardial ischemia. Methods 70 male Wistar rats were  randomly divided into fourteen group(n=5),control groups，model groups(2 h,4 h,6 h,12 h,24 h,48 h,72 h,1 week and 3 weeks after injection of isoproterenol hydrochloride(Iso))( the control group and 3 weeks group were divided into two groups)，positive control group and SF group. Except control group,all the rats in the other groups were treated with Iso (15 mg·kg^-1).In addition,the rats in positive control group were injected synchronously with bean and the rats in SF group were injected synchronously with SF. The tTG expression was observed before and after SF treatment by immunohistochemistry and RT-PCR. Results The tTG protein expression in rat ischemic myocardium 2 h after Iso injection was　gradually  increased 　compared with control group(P>0.05). With the prolongation of time of Iso injection,the tTG protein expression arrived a peak at 3 week compared with control group(P<0.01). The tTG mRNA expression at 24 h reached peak compared with control group and was  gradually decreased　at 48 h（P<0.05 or P<0.01）. The tTG protein expression after SF intervention was gradually decreased 　compared with model group(P<0.01). The tTG mRNA expression was also  gradually decreased　compared with model group（P<0.05 or P<0.01）.Conclusion The increased tTG expression accelerate the process of myocardial ischemia in rat myocardial ischemic model. SF can inhibit the tTG expression and reduce the process of myocardial ischemia.

Key words: myocardial fibrosis;tissue transglutaminases;sodium ferulate;disease model,animal