Abstract:

Objective To establish the high performane liqnid chromatography (HPLC)   for determining emodin in Gengnianan capsule，and provide the basis for the quality control of industrial production.Methods The HPLC was developed to determine the emodin in Gengnianan capsule.The developed conditions of HPLC were as follows: the Shimpack VP-ODS C18(150 mm×4.6mm,5 μm), methanol-0.1% phosphoric acid solution（77：23） as mobile phase,the detection wavelength of  254 nm,and the column temperature of 40℃.Results The linear range of emodin was 0.04-0.32 μg,the regression equation was Y=1060+2513102X，r=0.9991.The average recovery was 98.6%,and the RSD was 1.88% (n=6).The RSD in accuracy,stability and repeatability test were 0.86%,2.33% and 1.88%,respectively.Conclusion This method is accurate，simple and reliable,which provide the scientific basis for the quality control of Gengnianan capsule.

Key words: Gengnianan capsul；emodin;high performance liquid chromatography